2015年
Generation and characterization of MafA-Kusabira Orange mice
Endocrine Journal
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- 巻
- 62
- 号
- 1
- 開始ページ
- 37
- 終了ページ
- 51
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1507/endocrj.EJ14-0296
- 出版者・発行元
- Japan Endocrine Society
MafA and MafB are basic leucine zipper transcription factors expressed in mature pancreatic β- and α-cells, respectively. MafA is not only an insulin gene transcription factor but is also critical for the maturation and maintenance of β-cell function, whereas MafB is expressed in immature β-cells during development and in compromised β-cells in diabetes. In this study, we developed a mouse model to easily trace the promoter activity of MafA in β-cells as a tool for studying β-cell differentiation, maturation, regeneration and function using the expression of the fluorescent protein Kusabira Orange (KOr) driven by the BAC-mafA promoter. The expression of KOr was highly restricted to β-cells in the transgenic pancreas. By crossing MafA-KOr mice with MafBGFP/+ reporter mice, simultaneous monitoring of MafA and MafB expressions in the isolated islets was successfully performed. This system can be a useful tool for examining dynamic changes in the differentiation and function of pancreatic islets by visualizing the expressions of MafA and MafB.
- リンク情報
- ID情報
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- DOI : 10.1507/endocrj.EJ14-0296
- ISSN : 1348-4540
- ISSN : 0918-8959
- PubMed ID : 25273397
- SCOPUS ID : 84922056106