論文

査読有り
2015年

Generation and characterization of MafA-Kusabira Orange mice

Endocrine Journal
  • Wataru Nishimura
  • ,
  • Hisashi Oishi
  • ,
  • Nobuaki Funahashi
  • ,
  • Toshiyoshi Fujiwara
  • ,
  • Satoru Takahashi
  • ,
  • Kazuki Yasuda

62
1
開始ページ
37
終了ページ
51
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1507/endocrj.EJ14-0296
出版者・発行元
Japan Endocrine Society

MafA and MafB are basic leucine zipper transcription factors expressed in mature pancreatic β- and α-cells, respectively. MafA is not only an insulin gene transcription factor but is also critical for the maturation and maintenance of β-cell function, whereas MafB is expressed in immature β-cells during development and in compromised β-cells in diabetes. In this study, we developed a mouse model to easily trace the promoter activity of MafA in β-cells as a tool for studying β-cell differentiation, maturation, regeneration and function using the expression of the fluorescent protein Kusabira Orange (KOr) driven by the BAC-mafA promoter. The expression of KOr was highly restricted to β-cells in the transgenic pancreas. By crossing MafA-KOr mice with MafBGFP/+ reporter mice, simultaneous monitoring of MafA and MafB expressions in the isolated islets was successfully performed. This system can be a useful tool for examining dynamic changes in the differentiation and function of pancreatic islets by visualizing the expressions of MafA and MafB.

リンク情報
DOI
https://doi.org/10.1507/endocrj.EJ14-0296
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25273397
ID情報
  • DOI : 10.1507/endocrj.EJ14-0296
  • ISSN : 1348-4540
  • ISSN : 0918-8959
  • PubMed ID : 25273397
  • SCOPUS ID : 84922056106

エクスポート
BibTeX RIS