論文

査読有り 筆頭著者 責任著者 国際誌
2019年2月27日

Functional crosstalk across IMD and Toll pathways: insight into the evolution of incomplete immune cascades.

Proceedings. Biological sciences
  • Yudai Nishide
  • ,
  • Daisuke Kageyama
  • ,
  • Kakeru Yokoi
  • ,
  • Akiya Jouraku
  • ,
  • Hiromitsu Tanaka
  • ,
  • Ryo Futahashi
  • ,
  • Takema Fukatsu

286
1897
開始ページ
20182207
終了ページ
20182207
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1098/rspb.2018.2207

In insects, antimicrobial humoral immunity is governed by two distinct gene cascades, IMD pathway mainly targeting Gram-negative bacteria and Toll pathway preferentially targeting Gram-positive bacteria, which are widely conserved among diverse metazoans. However, recent genomic studies uncovered that IMD pathway is exceptionally absent in some hemipteran lineages like aphids and assassin bugs. How the apparently incomplete immune pathways have evolved with functionality is of interest. Here we report the discovery that, in the hemipteran stinkbug Plautia stali, both IMD and Toll pathways are present but their functional differentiation is blurred. Injection of Gram-negative bacteria and Gram-positive bacteria upregulated effector genes of both pathways. Notably, RNAi experiments unveiled significant functional permeation and crosstalk between IMD and Toll pathways: RNAi of IMD pathway genes suppressed upregulation of effector molecules of both pathways, where the suppression was more remarkable for IMD effectors; and RNAi of Toll pathway genes reduced upregulation of effector molecules of both pathways, where the suppression was more conspicuous for Toll effectors. These results suggest the possibility that, in hemipterans and other arthropods, IMD and Toll pathways are intertwined to target wider and overlapping arrays of microbes, which might have predisposed and facilitated the evolution of incomplete immune pathways.

リンク情報
DOI
https://doi.org/10.1098/rspb.2018.2207
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30963836
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408883
ID情報
  • DOI : 10.1098/rspb.2018.2207
  • PubMed ID : 30963836
  • PubMed Central 記事ID : PMC6408883

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