2015年7月
Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation
Nature Structural & Molecular Biology
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- ,
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- 巻
- 22
- 号
- 7
- 開始ページ
- 572
- 終了ページ
- +
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nsmb.3036
- 出版者・発行元
- NATURE PUBLISHING GROUP
Atg101 is an essential component of the autophagy-initiating ULK complex in higher eukaryotes, but it is absent from the functionally equivalent Atg1 complex in budding yeast. Here, we report the crystal structure of the fission yeast Atg101-Atg13 complex. Atg101 has a Hop1, Rev7 and Mad2 (HORMA) architecture similar to that of Atg13. Mad2 HORMA has two distinct conformations (O-Mad2 and C-Mad2), and, intriguingly, Atg101 resembles O-Mad2 rather than the C-Mad2-like Atg13. Atg13 HORMA from higher eukaryotes possesses an inherently unstable fold, which is stabilized by Atg101 via interactions analogous to those between O-Mad2 and C-Mad2. Mutational studies revealed that Atg101 is responsible for recruiting downstream factors to the autophagosome-formation site in mammals via a newly identified WF finger. These data define the molecular functions of Atg101, providing a basis for elucidating the molecular mechanisms of mammalian autophagy initiation by the ULK complex.
- リンク情報
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- DOI
- https://doi.org/10.1038/nsmb.3036
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26030876
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000357614900009&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/26030876
- URL
- http://orcid.org/0000-0002-6940-8069
- ID情報
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- DOI : 10.1038/nsmb.3036
- ISSN : 1545-9993
- eISSN : 1545-9985
- ORCIDのPut Code : 20723639
- PubMed ID : 26030876
- Web of Science ID : WOS:000357614900009