2014年6月
Structural basis of starvation-induced assembly of the autophagy initiation complex
Nature Structural & Molecular Biology
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- 巻
- 21
- 号
- 6
- 開始ページ
- 513
- 終了ページ
- 521
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nsmb.2822
- 出版者・発行元
- NATURE PUBLISHING GROUP
Assembly of the preautophagosomal structure (PAS) is essential for autophagy initiation in yeast. Starvation-induced dephosphorylation of Atg13 is required for the formation of the Atg1-Atg13-Atg17-Atg29-Atg31 complex (Atg1 complex), a prerequisite for PAS assembly. However, molecular details underlying these events have not been established. Here we studied the interactions of yeast Atg13 with Atg1 and Atg17 by X-ray crystallography. Atg13 binds tandem microtubule interacting and transport domains in Atg1, using an elongated helix-loop-helix region. Atg13 also binds Atg17, using a short region, thereby bridging Atg1 and Atg17 and leading to Atg1-complex formation. Dephosphorylation of specific serines in Atg13 enhanced its interaction with not only Atg1 but also Atg17. These observations update the autophagy-initiation model as follows: upon starvation, dephosphorylated Atg13 binds both Atg1 and Atg17, and this promotes PAS assembly and autophagy progression.
- リンク情報
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- DOI
- https://doi.org/10.1038/nsmb.2822
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24793651
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000337010500005&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/24793651
- URL
- http://orcid.org/0000-0002-6940-8069
- ID情報
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- DOI : 10.1038/nsmb.2822
- ISSN : 1545-9993
- eISSN : 1545-9985
- ORCIDのPut Code : 20723642
- PubMed ID : 24793651
- Web of Science ID : WOS:000337010500005