論文

査読有り 国際誌
2018年11月

Epithelial-mesenchymal transition is activated in CD44-positive malignant ascites tumor cells of gastrointestinal cancer.

Cancer science
  • Michitaka Nakano
  • ,
  • Mamoru Ito
  • ,
  • Risa Tanaka
  • ,
  • Hiroshi Ariyama
  • ,
  • Kenji Mitsugi
  • ,
  • Akitaka Makiyama
  • ,
  • Keita Uchino
  • ,
  • Taito Esaki
  • ,
  • Nobuhiro Tsuruta
  • ,
  • Fumiyasu Hanamura
  • ,
  • Kyoko Yamaguchi
  • ,
  • Yuta Okumura
  • ,
  • Kosuke Sagara
  • ,
  • Kotoe Takayoshi
  • ,
  • Kenta Nio
  • ,
  • Kenji Tsuchihashi
  • ,
  • Shingo Tamura
  • ,
  • Hozumi Shimokawa
  • ,
  • Shuji Arita
  • ,
  • Kohta Miyawaki
  • ,
  • Hitoshi Kusaba
  • ,
  • Koichi Akashi
  • ,
  • Eishi Baba

109
11
開始ページ
3461
終了ページ
3470
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.13777

Disseminated cancer cells in malignant ascites possess unique properties that differ from primary tumors. However, the biological features of ascites tumor cells (ATC) have not been fully investigated. By analyzing ascites fluid from 65 gastrointestinal cancer patients, the distinguishing characteristics of ATC were identified. High frequency of CD44+ cells was observed in ATC using flow cytometry (n = 48). Multiplex quantitative PCR (n = 15) showed higher gene expression of epithelial-mesenchymal transition (EMT)-related genes and transforming growth factor beta (TGF-beta)-related genes in ATC than in the primary tissues. Immunohistochemistry (n = 10) showed that ATC also had much higher expression of phosphorylated SMAD2 than that in the corresponding primary tissues. TGF-beta 1 was detected in all cases of malignant ascites by enzyme-linked immunoassay (n = 38), suggesting the possible interaction of ATC and the ascites microenvironment. In vitro experiments revealed that these ATC properties were maintained by TGF-beta 1 in cultured ATC(n = 3). Here, we showed that ATCrevealed high frequencies of CD44 and possessed distinct EMT features from primary tissues that were mainly maintained by TGF-beta 1 in the ascites.

リンク情報
DOI
https://doi.org/10.1111/cas.13777
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30142697
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215886
ID情報
  • DOI : 10.1111/cas.13777
  • ISSN : 1347-9032
  • PubMed ID : 30142697
  • PubMed Central 記事ID : PMC6215886

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