論文

査読有り
2016年1月

The Dysferlin Domain-Only Protein, Spo73, Is Required for Prospore Membrane Extension in Saccharomyces cerevisiae

MSPHERE
  • Yuuya Okumura
  • ,
  • Tsuyoshi S. Nakamura
  • ,
  • Takayuki Tanaka
  • ,
  • Ichiro Inoue
  • ,
  • Yasuyuki Suda
  • ,
  • Tetsuo Takahashi
  • ,
  • Hideki Nakanishi
  • ,
  • Shugo Nakamura
  • ,
  • Xiao-Dong Gao
  • ,
  • Hiroyuki Tachikawa

1
1
開始ページ
e00038-15
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/mSphere.00038-15
出版者・発行元
AMER SOC MICROBIOLOGY

Sporulation of Saccharomyces cerevisiae is a developmental process in which an ascus containing four haploid spores forms from a diploid cell. During this process, newly formed membrane structures called prospore membranes extend along the nuclear envelope and engulf and package daughter nuclei along with cytosol and organelles to form precursors of spores. Proteins involved in prospore membrane extension, Vps13 and Spo71, have recently been reported; however, the overall mechanism of membrane extension remains unclear. Here, we identified Spo73 as an additional factor involved in prospore membrane extension. Analysis of a spo73 Delta mutant revealed that it shows defects similar to those of a spo71 Delta mutant during prospore membrane formation. Spo73 localizes to the prospore membrane, and this localization is independent of Spo71 and Vps13. In contrast, a Spo73 protein carrying mutations in a surface basic patch mislocalizes to the cytoplasm and overexpression of Spo71 can partially rescue localization to the prospore membrane. Similar to spo71 Delta mutants, spo73 Delta mutants display genetic interactions with the mutations in the SMA2 and SPO1 genes involved in prospore membrane bending. Further, our bioinformatic analysis revealed that Spo73 is a dysferlin domain-only protein. Thus, these results suggest that a dysferlin domain-only protein, Spo73, functions with a dual pleckstrin homology domain protein, Spo71, in prospore membrane extension. Analysis of Spo73 will provide insights into the conserved function of dysferlin domains, which is related to dysferlinopathy.
IMPORTANCE Prospore membrane formation consists of de novo double-membrane formation, which occurs during the developmental process of sporulation in Saccharomyces cerevisiae. Membranes are formed into their proper size and shape, and thus, prospore membrane formation has been studied as a general model of membrane formation. We identified SPO73, previously shown to be required for spore wall formation, as an additional gene involved in prospore membrane extension. Genetic and cell biological analyses suggested that Spo73 functions on the prospore membrane with other factors in prospore membrane extension, counteracting the bending force of the prospore membrane. Spo73 is the first dysferlin domain-only protein ever analyzed. The dysferlin domain is conserved from yeast to mammals and is found in dysferlin proteins, which are involved in dysferlinopathy, although the precise function of the domain is unknown. Continued analysis of Spo73 will contribute to our understanding of the function of dysferlin domains and dysferlinopathy.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1128/mSphere.00038-15
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27303688
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000392583700021&DestApp=WOS_CPL
URL
https://msphere.asm.org/content/1/1/e00038-15

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