論文

査読有り
2017年12月

Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation

MOLECULAR BIOLOGY OF THE CELL
  • Tsuyoshi S. Nakamura
  • ,
  • Yumi Numajiri
  • ,
  • Yuuya Okumura
  • ,
  • Junji Hidaka
  • ,
  • Takayuki Tanaka
  • ,
  • Ichiro Inoue
  • ,
  • Yasuyuki Suda
  • ,
  • Tetsuo Takahashi
  • ,
  • Hideki Nakanishi
  • ,
  • Xiao-Dong Gao
  • ,
  • Aaron M. Neiman
  • ,
  • Hiroyuki Tachikawa

28
26
開始ページ
3881
終了ページ
3895
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1091/mbc.E17-08-0521
出版者・発行元
AMER SOC CELL BIOLOGY

During the developmental process of sporulation in Saccharomyces cerevisiae, membrane structures called prospore membranes are formed de novo, expand, extend, acquire a round shape, and finally become plasma membranes of the spores. GIP1 encodes a regulatory/targeting subunit of protein phosphatase type 1 that is required for sporulation. Gip1 recruits the catalytic subunit Glc7 to septin structures that form along the prospore membrane; however, the molecular basis of its localization and function is not fully understood. Here we show that Gip1 changes its localization dynamically and is required for prospore membrane extension. Gip1 first associates with the spindle pole body as the prospore membrane forms, moves onto the prospore membrane and then to the septins as the membrane extends, distributes around the prospore membrane after closure, and finally translocates into the nucleus in the maturing spore. Deletion and mutation analyses reveal distinct sequences in Gip1 that are required for different localizations and for association with Glc7. Binding to Glc7 is also required for proper localization. Strikingly, localization to the prospore membrane, but not association with septins, is important for Gip1 function. Further, our genetic analysis suggests that a Gip1-Glc7 phosphatase complex regulates prospore membrane extension in parallel to the previously reported Vps13, Spo71, Spo73 pathway.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1091/mbc.E17-08-0521
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29046399
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000417843200013&DestApp=WOS_CPL
URL
https://www.molbiolcell.org/doi/abs/10.1091/mbc.E17-08-0521?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed

エクスポート
BibTeX RIS