武井 延之

J-GLOBALへ         更新日: 19/09/24 17:30
 
アバター
研究者氏名
武井 延之
 
タケイ ノブユキ
所属
新潟大学
部署
脳研究所 基礎神経科学部門
職名
准教授
学位
理学修士(上智大学), 理学博士(上智大学)
その他の所属
新潟大学新潟大学
科研費研究者番号
70221372

研究分野

 
 

経歴

 
1998年7月
 - 
1999年3月
新潟大学 脳研究所 講師
 
1999年4月
 - 
2007年3月
新潟大学 脳研究所 基礎神経科学部門 助教授
 
2007年4月
 - 
現在
新潟大学 脳研究所 基礎神経科学部門 准教授
 
2007年4月
 - 
現在
新潟大学 医歯学総合研究科 分子細胞医学専攻 准教授
 
2007年4月
 - 
現在
新潟大学 医歯学総合研究科 医科学専攻 准教授
 

論文

 
Kobayashi Y, Iwakura Y, Sotoyama H, Kitayama E, Takei N, Someya T, Nawa H
Translational psychiatry   9(1) 181   2019年8月   [査読有り]
Yamada S, Kamata T, Nawa H, Sekijima T, Takei N
Scientific reports   9(1) 11904   2019年8月   [査読有り]
Miki D, Kobayashi Y, Okada T, Miyamoto T, Takei N, Sekino Y, Koganezawa N, Shirao T, Saito Y
Neurochemical research   44(7) 1736-1744   2019年7月   [査読有り]
Yukawa T, Iwakura Y, Takei N, Saito M, Watanabe Y, Toyooka K, Igarashi M, Niizato K, Oshima K, Kunii Y, Yabe H, Matsumoto J, Wada A, Hino M, Iritani S, Niwa SI, Takeuchi R, Takahashi H, Kakita A, Someya T, Nawa H
Psychiatry research   270 940-946   2018年12月   [査読有り]
Hoshi O, Sugizaki A, Cho Y and Takei N
Neurochem.Res.   43 1242-1249   2018年4月   [査読有り]
Furukawa K, Fuse I, Iwakura Y, Sotoyama H, Hanyu O, Nawa H, Sone H, Takei N
Cardiovascular diabetology   16(1) 20   2017年2月   [査読有り]
Iwakura Y, Wang R, Inamura N, Araki K, Higashiyama S, Takei N, Nawa N
PLoS One   12 e0174780   2017年   [査読有り]
Namba H, Nagano T, Jodo E, Eifuku S, Horie M, Takebayashi H, Iwakura Y, Sotoyama H, Takei N, Nawa H
J. Neurochem.   142 886-900   2017年   [査読有り]
Sotoyama H, Iwakura Y, Oda K, Sasaoka T, Takei N, Kakita A, Enomoto H, Parsadanian A, Nawa H
Neurosci.Lett.   654 99-106   2017年   [査読有り]
1) Nakashima M, Saitsu H, Takei N, Tohyama J, Kato M, Kitaura H, Shiina M, Shirouzu H, Masuda H, Watanabe K, Ohba C, Tsurusaki Y, Miyake N, Zheng YJ, Sato T, Takebayashi H, Ogata K, Kameyama S, Kakita A, Matsumoto N
Ann. Neurol   78(3) 375-386   2015年9月   [査読有り]
Takei N, Furukawa K, Hanyu O, Sone H, NawaH
Front. Psychol.      2014年   [査読有り][招待有り]
Takei Nobuyuki, Nawa Hiroyuki
Front Mol Neurosci   7 28-28   2014年   [査読有り][招待有り]
Target of rapamycin (TOR) was first identified in yeast as a target molecule of rapamycin, an anti-fugal and immunosuppressant macrolide compound. In mammals, its orthologue is called mammalian TOR (mTOR). mTOR is a serine/threonine kinase that c...
Ishizuka Yuta, Kakiya Naomasa, Witters Lee A, Oshiro Noriko, Shirao Tomoaki, Nawa Hiroyuki, Takei Nobuyuki
J Neurochem   127(1) 66-77   2013年10月
Growth factors and nutrients, such as amino acids and glucose, regulate mammalian target of rapamycin complex 1 (mTORC1) signaling and subsequent translational control in a coordinated manner. Brain-derived neurotrophic factor (BDNF), the most pro...
Wang Ran, Iwakura Yuriko, Araki Kazuaki, Keino-Masu Kazuko, Masu Masayuki, Wang Xue-yi, Takei Nobuyuki, Higashiyama Shigeki, Nawa Hiroyuki
Sci Rep   3 1402-1402   2013年
Neuregulin-1 binds to ErbB3 and ErbB4 and regulates cancer proliferation and differentiation. Neuregulin-1 had been suggested to also react with ErbB2, but this argument becomes controversial. Here, we re-evaluated the cellular responses and ErbB2...
Kakiya Naomasa, Saito Takashi, Nilsson Per, Matsuba Yukio, Tsubuki Satoshi, Takei Nobuyuki, Nawa Hiroyuki, Saido Takaomi C
J Biol Chem   287(35) 29362-29372   2012年8月
Neprilysin is one of the major amyloid-beta peptide (Abeta)-degrading enzymes, the expression of which declines in the brain during aging. The decrease in neprilysin leads to a metabolic Abeta imbalance, which can induce the amyloidosis underlying...
Kitaura Hiroki, Oishi Makoto, Takei Nobuyuki, Fu Yong-Juan, Hiraishi Tetsuya, Fukuda Masafumi, Takahashi Hitoshi, Shibuki Katsuei, Fujii Yukihiko, Kakita Akiyoshi
Epilepsia   53(7) e127-e131   2012年7月
Patients with periventricular nodular heterotopia (PVNH) often have severe epilepsy. However, it is unclear how the heterotopia contributes to epileptogenesis. Recently, electrophysiologic studies using intraoperative depth electrodes have indica...
Iwakura Yuriko, Zheng Yingjun, Sibilia Maria, Abe Yuichi, Piao Ying-Shan, Yokomaku Daisaku, Wang Ran, Ishizuka Yuta, Takei Nobuyuki, Nawa Hiroyuki
J Neurochem   118(1) 45-56   2011年7月
Although epidermal growth factor (EGF) receptor (ErbB1) is implicated in Parkinson's disease and schizophrenia, the neurotrophic action of ErbB1 ligands on nigral dopaminergic neurons remains controversial. Here, we ascertained colocalization of E...
Iwakura Yuriko, Wang Ran, Abe Yuichi, Piao Ying-Shan, Shishido Yuji, Higashiyama Shigeki, Takei Nobuyuki, Nawa Hiroyuki
J Neurochem   118(1) 57-68   2011年7月
Epidermal growth factor (EGF) and structurally related peptides promote neuronal survival and the development of midbrain dopaminergic neurons; however, the regulation of their production has not been fully elucidated. In this study, we found tha...
Wang Ran, Iwakura Yuriko, Araki Kazuaki, Sotoyama Hidekazu, Takei Nobuyuki, Nawa Hiroyuki
Neurosci Lett   497(2) 90-93   2011年6月
The individual biological activities of many neurotrophic factors and their variants, which are produced by alternative splicing and proteolytic processing, often remain to be characterized. Bacterial protein production combined with protein refo...
Qi Sihua, Mizuno Makoto, Yonezawa Kazuyoshi, Nawa Hiroyuki, Takei Nobuyuki
Neurosci Res   68(2) 88-93   2010年10月
Novel protein synthesis is an essential element of various learning paradigms. Although pharmacological and genetic strategies have indicated the importance of translational activation in learning, the specific signaling pathways that are activat...
Reijonen Sami, Kukkonen Jyrki P, Hyrskyluoto Alise, Kivinen Jenny, Kairisalo Minna, Takei Nobuyuki, Lindholm Dan, Korhonen Laura
Cell Mol Life Sci   67(11) 1929-1941   2010年6月
Accumulation of abnormal proteins and endoplasmic reticulum stress accompany neurodegenerative diseases including Huntington's disease. We show that the expression of mutant huntingtin proteins with extended polyglutamine repeats differentially af...
Sakai Yasuhiro, Tanaka Takayuki, Seki Masaaki, Okuyama Shinya, Fukuchi Takeo, Yamagata Kanato, Takei Nobuyuki, Nawa Hiroyuki, Abe Haruki
Neurosci Res   65(4) 319-325   2009年12月
The exact role of cyclooxygenase-2 (COX-2) in neurodegeneration of retinal ganglion cells (RGCs) in vivo following ischemia-reperfusion injury of the retina was unknown. We made transgenic mice in which the Thy-1.2 promoter drives the expression o...
Takei Nobuyuki, Kawamura Mihoko, Ishizuka Yuta, Kakiya Naomasa, Inamura Naoko, Namba Hisaaki, Nawa Hiroyuki
J Biol Chem   284(39) 26340-26348   2009年9月
The constitutive and activity-dependent components of protein synthesis are both critical for neural function. Although the mechanisms controlling extracellularly induced protein synthesis are becoming clear, less is understood about the molecula...
Ishizuka Yuta, Kakiya Naomasa, Nawa Hiroyuki, Takei Nobuyuki
J Neurochem   106(2) 934-942   2008年7月
p70S6 kinase is a multipotent kinase that phosphorylates substrates in response to extracellular stimuli. This kinase activity inhibits apoptosis, regulates cell size and controls translation. In the CNS, p70S6K also participates in synaptic plast...
Mizuno M, Kawamura H, Takei N, Nawa H
J Neural Transm   115(3) 521-530   2008年
Abnormality in cytokine signaling is implicated in the neuropathology of schizophrenia. Previously, we established an animal model for schizophrenia by administering epidermal growth factor (EGF) to neonatal rats. Here we investigated effects of t...
Sotoyama Hidekazu, Namba Hisaaki, Takei Nobuyuki, Nawa Hiroyuki
Psychopharmacology (Berl)   191(3) 783-792   2007年4月
RATIONALE: Abnormality in the neurotrophic factor for dopamine neurons, epidermal growth factor (EGF), is associated with schizophrenia. Thus, rats treated with EGF as neonates are used as a putative animal model for schizophrenia showing impaired...
Nagano Tadasato, Namba Hisaaki, Abe Yuichi, Aoki Hiroyuki, Takei Nobuyuki, Nawa Hiroyuki
Eur J Neurosci   25(2) 380-390   2007年1月
The ErbB1 ligand family includes epidermal growth factor (EGF), transforming growth factor-alpha (TGFalpha), heparin-binding EGF-like growth factor, amphiregulin and betacellulin. Previously, we demonstrated that TGFalpha decreases alpha-amino-3-h...
Taniguchi Naoko, Shinoda Yo, Takei Nobuyuki, Nawa Hiroyuki, Ogura Akihiko, Tominaga-Yoshino Keiko
Neurosci Lett   406(1-2) 38-42   2006年10月
For the analysis of the cellular mechanism underlying long-term synaptic plasticity, a model system that allows long-lasting pursuit is required. Previously we reported that, in hippocampal neurons under dissociated cell culture conditions, repeat...
Shishido Yuji, Tanaka Takayuki, Piao Ying-shan, Araki Kazuaki, Takei Nobuyuki, Higashiyama Shigeki, Nawa Hiroyuki
Biochem Biophys Res Commun   348(3) 963-970   2006年9月
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is initially produced as a membrane-anchored precursor (pro-HB-EGF) and subsequently liberated from the cell membrane through ectodomain shedding. Here, we characterized the mole...
Namba Hisaaki, Nagano Tadasato, Iwakura Yuriko, Xiong Huabao, Jourdi Hussam, Takei Nobuyuki, Nawa Hiroyuki
Mol Cell Neurosci   31(4) 628-641   2006年4月
In the developing neocortex, brain-derived neurotrophic factor (BDNF) exerts a trophic activity to increase the expression and channel activity of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptor subunits. He...
Piao Ying-Shan, Iwakura Yuriko, Takei Nobuyuki, Nawa Hiroyuki
Neurosci Lett   390(1) 21-24   2005年12月
Using two-site enzyme immunoassays, we measured protein levels of epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha), and heparin-binding epidermal growth factor (HB-EGF) in adult rat brain, and compared them with the phos...
Inamura Naoko, Nawa Hiroyuki, Takei Nobuyuki
J Neurochem   95(5) 1438-1445   2005年12月
The effects and signaling mechanisms of brain-derived neurotrophic factor (BDNF) on translation elongation were investigated in cortical neurons. BDNF increased the elongation rate approximately twofold, as determined by measuring the ribosomal t...
Seki Masaaki, Tanaka Takayuki, Sakai Yasuhiro, Fukuchi Takeo, Abe Haruki, Nawa Hiroyuki, Takei Nobuyuki
Neurochem Res   30(9) 1163-1170   2005年9月
Muller cells, the predominant glial cells in the retina, are thought to play important roles in the survival of retinal neurons. Previous studies have demonstrated that Muller cells express brain-derived neurotrophic factor (BDNF), which has a pr...
Tohmi Manavu, Tsuda Noriko, Mizuno Makoto, Takei Nobuyuki, Frankland Paul W, Nawa Hiroyuki
Behav Genet   35(5) 615-629   2005年9月
Epidermal growth factor (EGF) receptor (ErbB1) signals regulate dopaminergic development and function and are implicated in schizophrenia. We evaluated genetic effects on neurobehavioral changes induced by neonatal EGF administration, using four m...
Iwakura Yuriko, Piao Ying-Shan, Mizuno Makoto, Takei Nobuyuki, Kakita Akiyoshi, Takahashi Hitoshi, Nawa Hiroyuki
J Neurochem   93(4) 974-983   2005年5月
Epidermal growth factor (EGF) is a member of a structurally related family containing heparin-binding EGF-like growth factor (HB-EGF) and transforming growth factor alpha (TGFalpha) that exerts neurotrophic activity on midbrain dopaminergic neuron...
Yokomaku D, Jourdi H, Kakita A, Nagano T, Takahashi H, Takei N, Nawa H
Neuroscience   136(4) 1037-1047   2005年
Scaffolding proteins containing postsynaptic density-95/discs large/zone occludens-1 (PDZ) domains interact with synaptic receptors and cytoskeletal components and are therefore implicated in synaptic development and plasticity. Little is known, h...
Takei Nobuyuki, Inamura Naoko, Kawamura Mihoko, Namba Hisaaki, Hara Kenta, Yonezawa Kazuyoshi, Nawa Hiroyuki
J Neurosci   24(44) 9760-9769   2004年11月
In neurons, perisynaptic or dendritic translation is implicated in synapse-wide alterations of function and morphology triggered by neural activity. The molecular mechanisms controlling local translation activation, however, have yet to be elucid...
Seki Masaaki, Fukuchi Takeo, Tanaka Takayuki, Nawa Hiroyuki, Takei Nobuyuki, Abe Haruki
Jpn J Ophthalmol   48(5) 460-464   2004年9月
PURPOSE: In this study, we sought to elucidate changes in the levels of neurotrophin-3 (NT-3) in the rat retina throughout postnatal development and aging. METHODS: We demonstrated NT-3 localization in the retina by immunohistochemistry. Protein a...
Seki Masaaki, Tanaka Takayuki, Nawa Hiroyuki, Usui Tomoaki, Fukuchi Takeo, Ikeda Kazuhito, Abe Haruki, Takei Nobuyuki
Diabetes   53(9) 2412-2419   2004年9月
Although neurotrophins have been assessed as candidate therapeutic agents for neural complications of diabetes, their involvement in diabetic retinopathy has not been fully characterized. We found that the protein and mRNA levels of brain-derived ...
Jourdi Hussam, Iwakura Yuriko, Narisawa-Saito Mako, Ibaraki Kyoko, Xiong Huabao, Watanabe Masahiko, Hayashi Yasunori, Takei Nobuyuki, Nawa Hiroyuki
Dev Biol   263(2) 216-230   2003年11月
Postsynaptic molecules with PDZ domains (PDZ proteins) interact with various glutamate receptors and regulate their subcellular trafficking and stability. In rat neocortical development, the protein expression of AMPA-type glutamate receptor GluR...
Nagano T, Yanagawa Y, Obata K, Narisawa-Saito M, Namba H, Otsu Y, Takei N, Nawa H
Mol Cell Neurosci   24(2) 340-356   2003年10月
The regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors is implicated in synaptic plasticity. Although we have found that brain-derived neurotrophic factor (BDNF) triggers surface translocation of...
Inamura Naoko, Nawa Hiroyuki, Takei Nobuyuki
Neurosci Lett   346(1-2) 117-119   2003年7月
Regulated protein synthesis is critical for neural development, such as the formation of synapses and neural circuits and the modulation of synaptic plasticity. Protein synthesis is controlled by translation factors, including initiation, elongati...
Seki Masaaki, Nawa Hiroyuki, Fukuchi Takeo, Abe Haruki, Takei Nobuyuki
Invest Ophthalmol Vis Sci   44(7) 3211-3218   2003年7月
PURPOSE: This study sought to elucidate changes in the levels and distribution of brain-derived neurotrophic factor (BDNF) in the retina throughout aging and depending on visual experience. METHODS: Protein and mRNA levels of BDNF were quantified ...
Amada Naoki, Tezuka Tomoyuki, Mayeda Akila, Araki Kazuaki, Takei Nobuyuki, Todokoro Kazuo, Nawa Hiroyuki
J Biochem   133(5) 615-623   2003年5月
The crooked neck (crn) gene of Drosophila melanogaster encodes a scaffold protein carrying multiple tetratricopeptide repeat (TPR) motifs, and its mutation results in a reduction in the number of neuroblasts and lethality during larval stages. Her...
Inamura Naoko, Hoshino Shin-ichi, Uchiumi Toshio, Nawa Hiroyuki, Takei Nobuyuki
Brain Res Mol Brain Res   111(1-2) 165-174   2003年3月
Novel protein synthesis in the brain has been suggested to contribute to the formation of synapses and neural circuits during development and the modulation of long-term synaptic plasticity through life. However, cellular and subcellular distribu...
Yuhara Aki, Ishii Kyoko, Nishio Chika, Abiru Yasuhiro, Yamada Masashi, Nawa Hiroyuki, Hatanaka Hiroshi, Takei Nobuyuki
Biochem Biophys Res Commun   301(2) 344-349   2003年2月
Both nerve growth factor (NGF) and pituitary adenylate cyclase activating polypeptide (PACAP) have neurotrophic effects on basal forebrain cholinergic neurons. They promote differentiation, maturation, and survival of these cholinergic neurons in ...
Mizuno M, Yamada K, Takei N, Tran M H, He J, Nakajima A, Nawa H, Nabeshima T
Mol Psychiatry   8(2) 217-224   2003年2月
Brain-derived neurotrophic factor (BDNF) plays a critical role in synaptic plasticity such as long-term potentiation (LTP), a form of synaptic correlate of learning and memory. BDNF is also implicated in learning and memory. We have demonstrated ...
Namba H, Takei N, Nawa H
Neuroscience   122(3) 637-646   2003年
Transforming growth factor-alpha (TGFalpha), a member of the epidermal growth factor family, has neurotrophic actions on postmitotic neurons. We examined the chronic effects of TGFalpha on the electrophysiological properties of one type of GABAerg...
Hashimoto Ryota, Takei Nobuyuki, Shimazu Kazuhiro, Christ Lori, Lu Bai, Chuang De-Maw
Neuropharmacology   43(7) 1173-1179   2002年12月
Mechanisms underlying the therapeutic effects of lithium for bipolar mood disorder remain poorly understood. Recent studies demonstrate that lithium has neuroprotective actions against a variety of insults in vitro and in vivo. This study was unde...
Narisawa-Saito Mako, Iwakura Yuriko, Kawamura Meiko, Araki Kazuaki, Kozaki Shunji, Takei Nobuyuki, Nawa Hiroyuki
J Biol Chem   277(43) 40901-40910   2002年10月
In hippocampal neurons, the exocytotic process of alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA)-type glutamate receptors is known to depend on activation of N-methyl-d-aspartate channels and its resultant Ca(2+) influx from extr...
Seki Masaaki, Nawa Hiroyuki, Morioka Tetsuo, Fukuchi Takeo, Oite Takashi, Abe Haruki, Takei Nobuyuki
Neurosci Lett   329(2) 185-188   2002年8月
In the central nervous system (CNS), Thy-1 is expressed predominantly on neurons and serves as a specific marker for neurons. In the present study, we established a two-site enzyme-linked immunosorbent assay (ELISA) that detects trace amounts of ...
Ueda Masashi, Hirose Munetaka, Takei Nobuyuki, Ibuki Takae, Naruse Yoshihisa, Amaya Fumimasa, Ibata Yasuhiko, Tanaka Masaki
Neurosci Lett   328(2) 97-100   2002年8月
Acute burn injury is usually associated with pain in the injured and nearby areas. However, we have recently reported that a thoracic scald induces hindpaw hyperalgesia during the healing stage in rats. The present study investigated the cause of ...
Xiong H, Futamura T, Jourdi H, Zhou H, Takei N, Diverse-Pierluissi M, Plevy S, Nawa H
Neuropharmacology   42(7) 903-912   2002年6月
Neurotrophins jointly exert various functions in the nervous system, including neuronal differentiation, survival, and regulation of synaptic plasticity. However, the functional interactions of neurotrophins or mechanisms through which neurotrophi...
Nerve growth factor enhances neurotransmitter release from PC12 cells by increasing Ca(2+)-responsible secretory vesicles through the activation of mitogen-activated protein kinase and phosphatidylinositol 3-kinase.
Amino Shinya, Itakura Makoto, Ohnishi Hiroshi, Tsujimura Jun, Koizumi Shinichi, Takei Nobuyuki, Takahashi Masami
J Biochem   131(6) 887-894   2002年6月
Neurotrophins play important roles in the differentiation and survival of neurons during development, and in the regulation of synaptic transmission in adult brain. Brief treatment with nerve growth factor (NGF) enhances depolarization and ionomyc...
Nawa H, Takei N
Trends Neurosci   24(12) 683-4; discussion 684   2001年12月
Studies of the peripheral nervous system have led to the concept of target-derived neurotrophic support. Neurotrophic factors such as nerve growth factor are now known to act as retrograde trophic factors--retrophins--that are produced in the targ...
Yuhara A, Nishio C, Abiru Y, Hatanaka H, Takei N
Brain Res Dev Brain Res   131(1-2) 41-45   2001年11月
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the vasoactive intestinal polypeptide gene family (VIP) that was originally isolated from rat hypothalamus. The high affinity PACAP receptor, PAC1, is expressed in the basa...
Takei N, Kawamura M, Hara K, Yonezawa K, Nawa H
J Biol Chem   276(46) 42818-42825   2001年11月
The effects of neurotrophic factors on translational activation were investigated in cortical neurons. Brain-derived neurotrophic factor (BDNF) increased protein synthesis within 30 min, whereas insulin produced a weaker enhancement of protein syn...
Matsumoto T, Numakawa T, Adachi N, Yokomaku D, Yamagishi S, Takei N, Hatanaka H
J Neurochem   79(3) 522-530   2001年11月
Brain-derived neurotrophic factor (BDNF) has been reported to play an important role in neuronal plasticity. In this study, we examined the effect of BDNF on an activity-dependent synaptic function in an acute phase. First, we found that short-te...
Iwakura Y, Nagano T, Kawamura M, Horikawa H, Ibaraki K, Takei N, Nawa H
J Biol Chem   276(43) 40025-40032   2001年10月
The dynamics of alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA)-type glutamate receptors, as represented by their exocytosis, endocytosis and cytoskeletal linkage, has often been implicated in N-methyl-d-aspartate (NMDA)-dependen...
Numakawa T, Matsumoto T, Adachi N, Yokomaku D, Kojima M, Takei N, Hatanaka H
J Neurosci Res   66(1) 96-108   2001年10月
We reported previously that BDNF induced glutamate release was dependent on intracellular Ca(2+) but not extracellular Ca(2+) in cerebellar neurons (Numakawa et al., 1999). It was revealed that the release was through a non-exocytotic pathway (Tak...
Kojima M, Takei N, Numakawa T, Ishikawa Y, Suzuki S, Matsumoto T, Katoh-Semba R, Nawa H, Hatanaka H
J Neurosci Res   64(1) 1-10   2001年4月
To visualize the release dynamics of the brain-derived neurotrophic factor (BDNF) involved in neural plasticity, we constructed a plasmid encoding green fluorescent protein (GFP) fused with BDNF. First, several biological studies confirmed that th...
Korhonen L, Sjoholm U, Takei N, Kern M A, Schirmacher P, Castren E, Lindholm D
Eur J Neurosci   12(10) 3453-3461   2000年10月
Hepatocyte growth factor-scatter factor (HGF) is expressed in different parts of the nervous system, and has been shown to exhibit neurotrophic activity. Here we show that c-Met, the receptor for HGF, is expressed in developing rat hippocampus, ...
Molecular characterization of a novel gamma-glutamyl transpeptidase homologue found in rat brain.
Yamaguchi T, Takei N, Araki K, Ishii K, Nagano T, Ichikawa T, Kumanishi T, Nawa H
J Biochem   128(1) 101-106   2000年7月
A cDNA clone for a novel homologue to gamma-glutamyl transpeptidase (gamma-GTP), termed GTPH, was isolated from a rat brain expression cDNA library using antisera against total brain synaptosomal fractions. The cloned GTPH consists of 641 amino a...
Takei N, Torres E, Yuhara A, Jongsma H, Otto C, Korhonen L, Abiru Y, Skoglosa Y, Schutz G, Hatanaka H, Sofroniew M V, Lindholm D
Eur J Neurosci   12(7) 2273-2280   2000年7月
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the vasointestinal polypeptide gene family for which neurotrophic activity has been postulated. PACAP mRNA is expressed in the developing and adult hippocampus, which is the...
Numakawa T, Takei N, Hatanaka H
Neurosci Res   37(1) 59-65   2000年5月
We investigated the effects of brain-derived neurotrophic factor (BDNF) on aspartate release from cultured cerebellar neurons. This release occurred within 1 min after the addition of 100 ng/ml BDNF. The amount of aspartate released was less tha...
Numakawa T, Takei N, Yamagishi S, Sakai N, Hatanaka H
Brain Res   842(2) 431-438   1999年9月
Brain-derived neurotrophic factor (BDNF) has been suggested to play an important role in neuronal plasticity. In this study, we investigated the effects of BDNF on short-term transmitter release from cultured CNS neurons. Rapid and transient glu...
Regulation of nerve growth factor release by nitric oxide through cyclic GMP pathway in cortical glial cells.
Xiong H, Yamada K, Jourdi H, Kawamura M, Takei N, Han D, Nabeshima T, Nawa H
Mol Pharmacol   56(2) 339-347   1999年8月
In the present study, we found that S-nitroso-N-acetyl-DL-penicillamine, a spontaneous nitric oxide (NO) generator, dose-dependently inhibited basal nerve growth factor (NGF) release from mixed glial cells. To elucidate the function of endogenous...
Skoglosa Y, Lewen A, Takei N, Hillered L, Lindholm D
Neuroscience   90(1) 235-247   1999年4月
Neurotrophic factors are known to promote neuronal survival during development and after acute brain injury. Recent data suggest that some neuropeptides also exhibit neurotrophic activities, as shown for the pituitary adenylate cyclase activating ...
Skoglosa Y, Takei N, Lindholm D
Brain Res Mol Brain Res   65(1) 1-13   1999年2月
PACAP is a member of the secretin/vasoactive intestinal peptide (VIP) family, isolated from hypothalamus. Recent studies have shown that PACAP is expressed in many parts of adult brain. We have studied the precise distribution of PACAP mRNA in de...
Takei N, Tanaka O, Endo Y, Lindholm D, Hatanaka H
Neuropharmacology   38(2) 283-288   1999年2月
The effect of neurotrophic factors on apoptosis induced by ionomycin, a potent Ca2+ ionophore, was investigated using cultured cortical neurons from embryonic rats. Brain-derived neurotophic factor (BDNF) and neurotrophin-3 (NT-3) prevented the io...
Lindholm D, Skoglosa Y, Takei N
Ann N Y Acad Sci   865 189-196   1998年12月
To function as a trophic factor PACAP and PACAP-R must be expressed in the nervous system during early development. We report here on the distribution of PACAP mRNA in the developing nervous system of the rat and compare its expression with that o...
Takei N, Skoglosa Y, Lindholm D
J Neurosci Res   54(5) 698-706   1998年12月
The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) is present in many regions of the adult and developing brain as are receptors for PACAP. PACAP stimulates different signalling cascades in neurons, involving cAMP, MAP kin...
Takei N, Numakawa T, Kozaki S, Sakai N, Endo Y, Takahashi M, Hatanaka H
J Biol Chem   273(42) 27620-27624   1998年10月
There is increasing interest in the involvement of neurotrophins in neural transmission and plasticity. Thus, we investigated the effects of brain-derived neurotrophic factor (BDNF) on glutamate release from cortical neurons. Treatment of culture...
Roles of neurotrophins on synaptic development and functions in the central nervous system.
Takei N, Nawa H
Hum Cell   11(3) 157-165   1998年9月
Evidence is emerging to suggest that in addition to their "classical" neurotrophic involvement in the regulation of the differentiation, maturation and survival of neurons, neurotrophins play crucial roles in neural transmission and succeeding ac...
Lioudyno M, Skoglosa Y, Takei N, Lindholm D
J Neurosci Res   51(2) 243-256   1998年1月
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a recently discovered neuropeptide which is present both in the central and peripheral nervous system of adult rats. Here we show that PACAP is also expressed by dorsal root ganglion se...
Shimoke K, Kubo T, Numakawa T, Abiru Y, Enokido Y, Takei N, Ikeuchi T, Hatanaka H
Brain Res Dev Brain Res   101(1-2) 197-206   1997年7月
Cerebellar granule neurons obtained from 9-day-old rats die in an apoptotic manner when cultured in serum-free medium containing a low concentration of potassium (5 mM). A high concentration of potassium (26 mM) in the culture medium and BDNF can ...
Takei N, Kuramoto H, Endo Y, Hatanaka H
Neurosci Lett   226(3) 207-209   1997年5月
The expression of vesicular acetylcholine transporter (VAChT), which transports ACh into synaptic vesicles, is coregulated with choline acetyltransferase (ChAT). Therefore, the effects of nerve growth factor (NGF) and brain-derived neurotrophic fa...
Maekawa S, Kumanogoh H, Funatsu N, Takei N, Inoue K, Endo Y, Hamada K, Sokawa Y
Biochim Biophys Acta   1323(1) 1-5   1997年1月
NAP-22 is a membrane-localized brain enriched acidic protein having a Ca(2+)-dependent calmodulin binding activity. Further fractionation of the NAP-22 containing membrane showed the localization of NAP-22 in a Triton insoluble fraction of low den...
Brain-derived neurotrophic factor increases the stimulation-evoked release of glutamate and the levels of exocytosis-associated proteins in cultured cortical neurons from embryonic rats.
Takei N, Sasaoka K, Inoue K, Takahashi M, Endo Y, Hatanaka H
J Neurochem   68(1) 370-375   1997年1月
Differentiation and survival of neurons induced by neurotrophins have been widely investigated, but little has been reported about the long-term effect of brain-derived neurotrophic factor (BDNF) on synaptic transmission. Among many steps of neuro...
Hatanaka Y, Suzuki K, Kawasaki Y, Endo Y, Taniguchi N, Takei N
Biochem Biophys Res Commun   227(2) 513-518   1996年10月
The implication of reactive oxygen species for the Ca2+ ionophore ionomycin-induced apoptosis was investigated in cultured cortical neurons from embryonic rats. Ionomycin increased the production of intracellular peroxides as measured by flow cyt...
Takei N, Sasaoka K, Higuchi H, Endo Y, Hatanaka H
Brain Res Mol Brain Res   37(1-2) 283-289   1996年4月
The effects of neurotrophic factor on the expression of neuropeptide Y (NPY) mRNA and on morphology of NPY-immunoreactive neurons were investigated. Brain-derived neurotrophic factor (BDNF) increased the expression of NPY mRNA in cultured cortica...
Takei N, Ogaki H, Endo Y
Neurosci Lett   192(2) 124-126   1995年6月
The effect of basic fibroblast growth factor (bFGF) on apoptotic cell death of cultured cortical neurons from embryonic rats induced by ionomycin, a potent Ca2+ ionophore, was investigated. bFGF inhibited Ca2+ ionophore-induced neurotoxicity in a...
Hattori T, Takei N, Mizuno Y, Kato K, Kohsaka S
Neurosci Res   21(3) 191-198   1995年1月
We previously reported that the gamma gamma-isozyme of enolase, NSE), one of the glycolytic enzymes, promoted the survival of embryonic rat neocortical neurons in culture, but alpha alpha-isozyme (non-neuronal enolase) had no effect. In the prese...
Takei N, Endo Y
Brain Res   652(1) 65-70   1994年7月
The neurotoxicity of ionomycin, a Ca2+ ionophore, was investigated in cultured cortical neurons from embryonic rats. While about 90% of neurons survived 2 h after exposure to ionomycin, the surviving neurons had decreased by about 30 to 40% at 16 ...
Takei N, Ohsawa K, Imai Y, Nakao H, Iwasaki A, Kohsaka S
Neurosci Lett   171(1-2) 59-62   1994年4月
Recombinant human annexin V showed survival promoting activity in embryonic rat neocortical and mesencephalic neurons in vitro. The neurotrophic effect was observed from a relatively low dose and in a dose-dependent manner. The neurotrophic activi...
Nagata K, Takei N, Nakajima K, Saito H, Kohsaka S
J Neurosci Res   34(3) 357-363   1993年2月
We previously reported that microglial conditioned medium (Mic-CM) has a neurotrophic effect on cultured rat neocortical neurons [Nakajima et al. (1989): Biomed Res 10:411-423]. In order to investigate the interaction between microglia and neuron...
Takei N, Kondo J, Nagaike K, Ohsawa K, Kato K, Kohsaka S
J Neurochem   57(4) 1178-1184   1991年10月
Neuronal survival factors in the central nervous system were investigated by using a primary culture of embryonic rat neocortical neurons. Bovine hippocampus was homogenized, and the supernatant from high-speed centrifugation was used as the star...
Production of basic fibroblast growth factor in cultured rat brain microglia.
Shimojo M, Nakajima K, Takei N, Hamanoue M, Kohsaka S
Neurosci Lett   123(2) 229-231   1991年2月
The production of basic fibroblast growth factor (bFGF) in cultured rat brain microglia was investigated. Rat brain microglia were found to express mRNA of bFGF in analysis by polymerase chain reaction (PCR) technique. Basic FGF was also detected ...
Interleukin-6 improves the survival of mesencephalic catecholaminergic and septal cholinergic neurons from postnatal, two-week-old rats in cultures.
Hama T, Kushima Y, Miyamoto M, Kubota M, Takei N, Hatanaka H
Neuroscience   40(2) 445-452   1991年
Interleukin-6 (human recombinant) supported the survival of cultured mesencephalic, catecholaminergic and septal cholinergic neurons from postnatal, two-week-old (P13-P15) rats. Significantly, more catecholaminergic neurons, stained by monoclonal ...
Monitoring of acetylcholine released from postnatal rat basal forebrain cholinergic neurons cultured on membrane filter by cell bed perfusion system and HPLC-ECD.
Takei N, Tsukui H, Kumakura K, Hatanaka H
Exp Neurol   108(3) 229-231   1990年6月
A method for the determination of release of endogenous neurotransmitter from cultured neurons has been developed. Basal forebrain cholinergic neurons from postnatal rats were cultured on polyethyleneimine-coated membrane filter with nerve growth ...
Increase in choline acetyltransferase activity in septum of rats after transient forebrain ischemia: a possible role of factors released in the hippocampus.
Hisanaga K, Kogure K, Tsukui H, Takei N, Nishio C, Hatanaka H
Neurosci Lett   105(3) 321-325   1989年11月
Choline acetyltransferase (ChAT) activity increased in rat septum 2 weeks after a transient forebrain ischemia. Extracts were prepared from hippocampus in which CA1 pyramidal neurons had been selectively destroyed by the ischemic insult. ChAT acti...
Takei N, Tsukui H, Hatanaka H
J Neurochem   53(5) 1405-1410   1989年11月
Cholinergic neurons from the septum area, the vertical limb of the diagonal band of Broca, and the nucleus basalis of Meynert of postnatal 13-day-old rats were cultured with or without nerve growth factor (NGF) conditions. Total choline acetyltra...
Transplantation of septal cholinergic neurons to the hippocampus improves memory impairments of spatial learning in rats treated with AF64A.
Ikegami S, Nihonmatsu I, Hatanaka H, Takei N, Kawamura H
Brain Res   496(1-2) 321-326   1989年9月
Embryonic septal neurons were transplanted into damaged hippocampus in adult rats which had received lateral ventricular administration of AF64A, a cholinergic neurotoxin. About 3 months after transplantation, the rats with bilateral septal graft...
Age-related decline of acetylcholine release evoked by depolarizing stimulation.
Takei N, Nihonmatsu I, Kawamura H
Neurosci Lett   101(2) 182-186   1989年6月
Release of endogenous acetylcholine (ACh) from the dorsal hippocampus in response to depolarizing stimulation with high-K+ infusion was examined in young and aged rats using the method of in vivo dialysis. ACh content in the dialysate was determi...
Recovery of hippocampal cholinergic activity by transplantation of septal neurons in AF64A treated rats.
Ikegami S, Nihonmatsu I, Hatanaka H, Takei N, Kawamura H
Neurosci Lett   101(1) 17-22   1989年6月
Embryonic septal neurons were transplanted into the hippocampus of adult rats which had received lateral-ventricular administration of AF64A, a cholinergic neurotoxin, and the effects on hippocampal cholinergic activity were studied. One week afte...
Transplantation of fetal and early postnatal rat septal cholinergic neurons cultured in serum-free and serum-containing medium with nerve growth factor.
Hisanaga K, Tsukui H, Takei N, Hatanaka H
Brain Res   475(2) 349-355   1988年12月
Fetal rat (E17-E19) septal neurons were cultured in a defined, serum-free medium for 6-8 days with or without nerve growth factor (NGF) and transplanted into the hippocampus or the surrounding ventricle of 28 adult rats denervated of its septal ...
Takei N, Tsukui H, Hatanaka H
J Neurochem   51(4) 1118-1125   1988年10月
The effects of nerve growth factor (NGF) on the intracellular content of acetylcholine (ACh) in cultured septal neurons from developing rats have been examined. The content of ACh could be measured by using HPLC and electrochemical detection (HPL...

Misc

 
シナプスにおける局所的翻訳調節機構
武井延之
生化学   78 939-948   2006年   [査読有り][依頼有り]
武井延之
化学と生物   46 24-31   2008年   [依頼有り]
統合失調症とマイクロRNA
渡部雄一郎、武井延之、那波宏之、染矢俊幸
分子精神医学   11 179-184   2011年7月   [依頼有り]
「基礎の基礎」神経細胞特異的翻訳調節機構
武井延之
細胞工学   31 642-645   2012年6月   [依頼有り]
「翻訳調節と脳高次機能」 神経特異的翻訳調節機構
武井延之
細胞工学   31 687-691   2012年6月   [依頼有り]

書籍等出版物

 
神経可塑性とサイトカイン in 脳・神経科学の最先端
那波宏之,斎藤真子,大津洋,熊化保,武井延之 (担当:共著, 範囲:7)
羊土社   1999年12月   
脳神経科学イラストレイテッド
斎藤真子,武井延之 (担当:共著, 範囲:9)
羊土社   2000年12月   
サイトカイン・増殖因子 用語ライブラリー
武井延之 (担当:共著, 範囲:3)
羊土社 東京   2005年12月   
「RNAと生命」
武井延之 (担当:共著, 範囲:7)
共立出版 東京   2006年12月   
「細胞増殖因子と再生医療」
那波宏之,武井延之 (担当:共著, 範囲:6)
メディカルレビュー社 大阪   2006年12月   

講演・口頭発表等

 
mTOR mediates neuronal maturation and cell size control [招待有り]
Takei N
ISN Satellite Symposium:Key molecules for neuronal maturation   2014年9月23日   ISN(International Society for Neurochemistry) /NIHS(National Institute of Health Sciences)
mTORシグナルと脳神経発達 [招待有り]
武井延之
第44回日本神経精神薬理学会大会   2014年11月20日   日本神経精神薬理学会
EGF downregulates presynaptic maturation and suppresses synapse formation in vitro and in vivo.
Takei N, Yokomaku D, Ushiki T, Nawa H
25th ISN Biennial Meeting   2015年8月23日   ISN (International Society for Neurochemistry)
mTORシグナル系と脳形成異常 [招待有り]
武井延之
BMB2015(日本生化学会/日本分子生物学会合同年会)   2015年12月1日   日本生化学会/日本分子生物学会
Epidermal growth factor downregulates presynaptic maturation and suppresses synapse formation in vitro and in vivo.
Takei N, Yokomaku D, Ushiki D and Nawa H
7th ISN Special Conference   2016年6月1日   International Society for Neurochemistry

競争的資金等の研究課題

 
遺伝性精神遅滞症脆弱X症候群の分子機構解析とその治療への応用
厚生労働省: 厚生労働科学研究費補助金
研究期間: 2001年4月 - 2003年3月    代表者: 塩見春彦
刺激に応答したmRNA翻訳マシナリーの神経突起への移動と活性化機構
文部科学省: 科学研究費助成事業
研究期間: 2003年4月 - 2004年3月
神経活動に依存した翻訳調節とその生理的意義に関する研究
日本学術振興会: 科学研究費助成事業
研究期間: 2003年4月 - 2006年3月
局所的mRNA翻訳マシナリーの活性化と脳高次機能
文部科学省: 科学研究費助成事業
研究期間: 2005年4月 - 2007年3月
中枢神経細胞における軸索内局所的蛋白合成の分子メカニズム
日本学術振興会: 科学研究費助成事業
研究期間: 2010年4月 - 2013年3月    代表者: 武井延之

委員歴

 
2001年
 - 
現在
日本神経化学会  評議員
 
2013年1月
 - 
現在
国際放射線神経生物学会  理事
 

社会貢献活動

 
Frontiers in Molecular Neuroscience誌 Editorial Board Member
【編集】  2019年 - 現在
Neurochemical Research誌 編集委員
【編集】  2009年 - 2015年
Scientific Reports 誌 Handling Editor
【編集】  2011年 - 現在
新発田高校学校評議員
【助言・指導】  新潟県  2018年4月 - 現在