論文

査読有り
2013年2月

Stemness-related Factor Sall4 Interacts with Transcription Factors Oct-3/4 and Sox2 and Occupies Oct-Sox Elements in Mouse Embryonic Stem Cells

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Nobuyuki Tanimura
  • ,
  • Motoki Saito
  • ,
  • Miki Ebisuya
  • ,
  • Eisuke Nishida
  • ,
  • Fuyuki Ishikawa

288
7
開始ページ
5027
終了ページ
5038
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M112.411173
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

A small number of transcription factors, including Oct-3/4 and Sox2, constitute the transcriptional network that maintains pluripotency in embryonic stem (ES) cells. Previous reports suggested that some of these factors form a complex that binds the Oct-Sox element, a composite sequence consisting of closely juxtaposed Oct-3/4 binding and Sox2 binding sites. However, little is known regarding the components of the complex. In this study we show that Sall4, a member of the Spalt-like family of proteins, directly interacts with Sox2 and Oct-3/4. Sall4 in combination with Sox2 or Oct-3/4 simultaneously occupies the Oct-Sox elements in mouse ES cells. Overexpression of Sall4 in ES cells increased reporter activities in a luciferase assay when the Pou5f1- or Nanog-derived Oct-Sox element was included in the reporter. Microarray analyses revealed that Sall4 and Sox2 bound to the same genes in ES cells significantly more frequently than expected from random coincidence. These factors appeared to bind the promoter regions of a subset of the Sall4 and Sox2 double-positive genes in precisely similar distribution patterns along the promoter regions, suggesting that Sall4 and Sox2 associate with such Sall4/Sox2-overlapping genes as a complex. Importantly, gene ontology analyses indicated that the Sall4/Sox2-overlapping gene set is enriched for genes involved in maintaining pluripotency. Sall4/Sox2/Oct-3/4 triple-positive genes identified by referring to a previous study identifying Oct3/4-bound genes in ES cells were further enriched for pluripotency genes than Sall4/Sox2 double-positive genes. These results demonstrate that Sall4 contributes to the transcriptional network operating in pluripotent cells together with Oct-3/4 and Sox2.

Web of Science ® 被引用回数 : 28

リンク情報
DOI
https://doi.org/10.1074/jbc.M112.411173
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23269686
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000315340400049&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.M112.411173
  • ISSN : 0021-9258
  • PubMed ID : 23269686
  • Web of Science ID : WOS:000315340400049

エクスポート
BibTeX RIS