2014年12月10日
Influenza virus-host interactome screen as a platform for antiviral drug development.
Cell host & microbe
- 巻
- 16
- 号
- 6
- 開始ページ
- 795
- 終了ページ
- 805
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.chom.2014.11.002
- 出版者・発行元
- CELL PRESS
Host factors required for viral replication are ideal drug targets because they are less likely than viral proteins to mutate under drug-mediated selective pressure. Although genome-wide screens have identified host proteins involved in influenza virus replication, limited mechanistic understanding of how these factors affect influenza has hindered potential drug development. We conducted a systematic analysis to identify and validate host factors that associate with influenza virus proteins and affect viral replication. After identifying over 1,000 host factors that coimmunoprecipitate with specific viral proteins, we generated a network of virus-host protein interactions based on the stage of the viral life cycle affected upon host factor downregulation. Using compounds that inhibit these host factors, we validated several proteins, notably Golgi-specific brefeldin A-resistant guanine nucleotide exchange factor 1 (GBF1) and JAK1, as potential antiviral drug targets. Thus, virus-host interactome screens are powerful strategies to identify targetable host factors and guide antiviral drug development.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.chom.2014.11.002
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/25464832
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451456
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000346211100013&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1016/j.chom.2014.11.002
- ISSN : 1931-3128
- eISSN : 1934-6069
- PubMed ID : 25464832
- PubMed Central 記事ID : PMC4451456
- Web of Science ID : WOS:000346211100013