2012年3月20日
F1Fo-ATPase, F-type proton-translocating ATPase, at the plasma membrane is critical for efficient influenza virus budding.
Proceedings of the National Academy of Sciences of the United States of America
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- 巻
- 109
- 号
- 12
- 開始ページ
- 4615
- 終了ページ
- 20
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.1114728109
- 出版者・発行元
- NATL ACAD SCIENCES
The identification of host factors involved in virus replication is important to understand virus life cycles better. Accordingly, we sought host factors that interact with the influenza viral nonstructural protein 2 by using coimmunoprecipitation followed by mass spectrometry. Among proteins associating with nonstructural protein 2, we focused on the β subunit of the F1Fo-ATPase, which received a high probability score in our mass spectrometry analysis. The siRNA-mediated down-regulation of the β subunit of the F1Fo-ATPase reduced influenza virion formation and virus growth in cell culture. We further found that efficient influenza virion formation requires the ATPase activity of F1Fo-ATPase and that plasma membrane-associated, but not mitochondrial, F1Fo-ATPase is important for influenza virion formation and budding. Hence, our data identify plasma membrane-associated F1Fo-ATPase as a critical host factor for efficient influenza virus replication.
- リンク情報
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- DOI
- https://doi.org/10.1073/pnas.1114728109
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22393008
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3311379
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000301712600055&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1073/pnas.1114728109
- ISSN : 0027-8424
- PubMed ID : 22393008
- PubMed Central 記事ID : PMC3311379
- Web of Science ID : WOS:000301712600055