論文

査読有り
2015年1月

Novel biological activity of ameloblastin in enamel matrix derivative

JOURNAL OF APPLIED ORAL SCIENCE
  • Sachiko Kuramitsu-Fujimoto
  • ,
  • Wataru Ariyoshi
  • ,
  • Noriko Saito
  • ,
  • Toshinori Okinaga
  • ,
  • Masaharu Kamo
  • ,
  • Akira Ishisaki
  • ,
  • Takashi Takata
  • ,
  • Kazunori Yamaguchi
  • ,
  • Tatsuji Nishihara

23
1
開始ページ
49
終了ページ
55
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1590/1678-775720140291
出版者・発行元
UNIV SAO PAULO FAC ODONTOLOGIA BAURU

Objective: Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration. However, the effects of EMD on gingival epithelial cells during regeneration of periodontal tissues are unclear. In this in vitro study, we purified ameloblastin from EMD and investigated its biological effects on epithelial cells. Material and Methods: Bioactive fractions were purified from EMD by reversed-phase high-performance liquid chromatography using hydrophobic support with a C18 column. The mouse gingival epithelial cell line GE-1 and human oral squamous cell carcinoma line SCC-25 were treated with purified EMD fraction, and cell survival was assessed with a WST-1 assay. To identify the proteins in bioactive fractions of EMD, we used proteome analysis with two-dimensional gel electrophoresis followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Results: Purified fractions from EMD suppressed proliferation of GE-1 and SCC-25. LC-MS/MS revealed that ameloblastin in EMD is the component responsible for inhibiting epithelial cell proliferation. The inhibitory effect of ameloblastin on the proliferation of GE-1 and SCC-25 was confirmed using recombinant protein. Conclusion: The inhibitory effects of EMD on epithelial cell proliferation are caused by the biological activities of ameloblastin, which suggests that ameloblastin is involved in regulating epithelial downgrowth in periodontal tissues.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1590/1678-775720140291
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000351541300009&DestApp=WOS_CPL