Papers

International journal
Dec, 2017

Cerebrospinal fluid injection into adult zebrafish for disease research.

Journal of neural transmission (Vienna, Austria : 1996)
  • Hideaki Matsui
  • ,
  • Noriko Matsui

Volume
124
Number
12
First page
1627
Last page
1633
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1007/s00702-017-1787-7

A modified method of cerebrospinal fluid injection was developed for the efficient and reliable administration of substances to the zebrafish central nervous system. The accuracy of this modified method was evaluated using Alexa Fluor dye injection. A high survival ratio was achieved due to the simplicity of the procedure and ice-tricaine combined anaesthesia. To validate this new method, we injected ammonium chloride, which successfully blocked lysosome function resulting in elevated LC3-II and the accumulation of ubiquitinated proteins. Injection of human α-synuclein fibrils initiated a prion-like propagation of α-synuclein pathology in zebrafish. This method can be used to investigate the effects of various substances and the propagation of α-synuclein in the central nervous system.

Link information
DOI
https://doi.org/10.1007/s00702-017-1787-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28865043
ID information
  • DOI : 10.1007/s00702-017-1787-7
  • Pubmed ID : 28865043

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