2017年3月
Phloridzin inhibits high K+-induced contraction via the inhibition of sodium: glucose cotransporter 1 in rat ileum
JOURNAL OF VETERINARY MEDICAL SCIENCE
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- 巻
- 79
- 号
- 3
- 開始ページ
- 593
- 終了ページ
- 601
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1292/jvms.16-0560
- 出版者・発行元
- JAPAN SOC VET SCI
Recent studies have shown that phloridzin, an inhibitor of sodium glucose cotransporter (SGLT), strongly decreases high K+-induced contraction in phasic muscle, such as tenia coil, but slightly affects tonic muscle, such as trachea. In this study, we examined the inhibitory mechanism of phloridzin on high K+-induced muscle contraction in rat ileum, a phasic muscle. Phloridzin inhibited the high K+-induced contraction in the ileum and the aorta, and the relaxing effect of phloridzin at 1 mM in the ileum was approximately five-fold more potent than that in the aorta. The expression of SGLT1 mRNA in the ileum was higher than that of the aorta. Phloridzin significantly inhibited NADH/NAD ratio and phosphocreatine (PCr) content in the ileum; I however, application of pyruvate recovered the inhibition of contraction and PCr content, but had no effect on ratio of NADH/NAD. High K+ increased 2-(N (7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) uptake in ileal smooth muscle cells, and phloridzin inhibited the increase in a concentration-dependent manner. These results suggest that phloridzin inhibits high K+-induced contraction because of the inhibition of energy metabolism via the inhibition of SGLT1.
- リンク情報
- ID情報
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- DOI : 10.1292/jvms.16-0560
- ISSN : 0916-7250
- eISSN : 1347-7439
- PubMed ID : 28190822
- Web of Science ID : WOS:000397830400024