2015年8月
Xist Exon 7 Contributes to the Stable Localization of Xist RNA on the Inactive X-Chromosome
PLOS GENETICS
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- 巻
- 11
- 号
- 8
- 開始ページ
- e100543
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pgen.1005430
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
To equalize X-linked gene dosage between the sexes in mammalian females, Xist RNA inactivates one of the two X-chromosomes. Here, we report the crucial function of Xist exon 7 in X-inactivation. Xist exon 7 is the second-largest exon with a well-conserved repeat E in eutherian mammals, but its role is often overlooked in X-inactivation. Although female ES cells with a targeted truncation of the Xist exon 7 showed no significant differences in their Xist expression levels and RNA stability from control cells expressing wild-type Xist, compromised localization of Xist RNA and incomplete silencing of X-linked genes on the inactive X-chromosome (Xi) were observed in the exon 7-truncated mutant cells. Furthermore, the interaction between the mutant Xist RNA and hnRNP U required for localization of Xist RNA to the Xi was impaired in the Xist exon 7 truncation mutant cells. Our results suggest that exon 7 of Xist RNA plays an important role for stable Xist RNA localization and silencing of the X-linked genes on the Xi, possibly acting through an interaction with hnRNP U.
- リンク情報
- ID情報
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- DOI : 10.1371/journal.pgen.1005430
- ISSN : 1553-7404
- Web of Science ID : WOS:000360823100023