Odor sensing system with high sensitivity and selectivity is expected to be realized by using biological mechanisms such as olfactory receptor. We have already developed a cell-based odor sensing system which acquires odorant concentration as a fluorescent intensity change. In the measurement, however, an artifact is likely to happen due to lack of stable and reproducible methods for delivering odorants to biological cells. Therefore, we developed and evaluated the automated flow measurement system which can simultaneously control pump, solenoid valve, and camera. Our system realized automation of solution supply and fluorescent measurement. As a result, our system could reduce artifacts and repeatedly performed measurements with the same sensor cells.