論文

査読有り 国際誌
2018年11月30日

Electrophysiological measurement of ion channels on plasma/organelle membranes using an on-chip lipid bilayer system.

Scientific reports
  • Koki Kamiya
  • ,
  • Toshihisa Osaki
  • ,
  • Kenji Nakao
  • ,
  • Ryuji Kawano
  • ,
  • Satoshi Fujii
  • ,
  • Nobuo Misawa
  • ,
  • Masatoshi Hayakawa
  • ,
  • Shoji Takeuchi

8
1
開始ページ
17498
終了ページ
17498
記述言語
英語
掲載種別
DOI
10.1038/s41598-018-35316-4

Ion channels are located in plasma membranes as well as on mitochondrial, lysosomal, and endoplasmic reticulum membranes. They play a critical role in physiology and drug targeting. It is particularly challenging to measure the current mediated by ion channels in the lysosomal and the endoplasmic reticulum membranes using the conventional patch clamp method. In this study, we show that our proposed device is applicable for an electrophysiological measurement of various types of ion channel in plasma and organelle membranes. We designed an on-chip device that can form multiple electrical contacts with a measurement system when placed on a mount system. Using crude cell membranes containing ion channels extracted from cultured cells without detergents, we detected open/close signals of the hERG, TRPV1, and NMDA channels on plasma membranes, those of the TRPML1 channels on lysosomal membranes, and open/close signals of the RyR channels on SR membranes. This method will provide a highly versatile drug screening system for ion channels expressed by various cell membranes, including plasma, SR, mitochondrial, Golgi, and lysosomal membranes.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1038/s41598-018-35316-4
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30504856
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269590
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000451748700020&DestApp=WOS_CPL