論文

査読有り 国際誌
2016年3月

Activation of Wnt/ß-catenin signaling in ESC promotes rostral forebrain differentiation in vitro.

In vitro cellular & developmental biology. Animal
  • Nozomu Takata
  • ,
  • Eriko Sakakura
  • ,
  • Yoshiki Sasai

52
3
開始ページ
374
終了ページ
382
記述言語
英語
掲載種別
DOI
10.1007/s11626-015-9975-y

Wnt/ß-catenin signaling is crucial for maintenance of pluripotent state of embryonic stem cell (ESC). However, it is unclear how Wnt/ß-catenin signaling affects the differentiation ability of ESC, especially with regard to rostral forebrain cells. Here, using Rax, rostral forebrain marker, and Wnt/ß-catenin reporter lines, we report ratio of Rax(+) and Wnt responding tissue (Wnt(+)) patterns, which were affected by seeding number of ESC in three-dimensional culture system. Surprisingly, we found ß-catenin level and localization are heterogeneous in ESC colony by immunostaining and time-laps imaging of ß-catenin-mEGFP signals. Moreover, activation of Wnt signaling in ESC promoted expression level and nuclear localization of ß-catenin, and mRNA levels of Wnt antagonists, axin2 and dkk1, leading to upregulating Wnt/ß-catenin reporter in ESC state and Rax expression at differentiation culture day 7. Together, our results suggest that activation of Wnt signaling in ESC promotes the differentiation efficacy of rostral forebrain cells. Wnt-priming culture method may provide a useful tool for applications in the areas of basic science and molecular therapeutics for regenerative medicine.

リンク情報
DOI
https://doi.org/10.1007/s11626-015-9975-y
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26563247
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833820
ID情報
  • DOI : 10.1007/s11626-015-9975-y
  • PubMed ID : 26563247
  • PubMed Central 記事ID : PMC4833820

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