論文

査読有り 国際誌
2020年8月5日

Sustained laryngeal transient receptor potential vanilloid 1 activation inhibits mechanically-induced swallowing in anesthetized rats.

American journal of physiology. Gastrointestinal and liver physiology
  • Midori Yoshihara
  • ,
  • Takanori Tsujimura
  • ,
  • Taku Suzuki
  • ,
  • Kouta Nagoya
  • ,
  • Naru Shiraishi
  • ,
  • Jin Magara
  • ,
  • Miho Terunuma
  • ,
  • Makoto Inoue

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1152/ajpgi.00082.2020

A major component of gastric acid is hydrochloric acid (HCl), which can activate transient receptor potential vanilloid 1 (TRPV1). In the present study, we investigated how sustained laryngeal TRPV1 activation affects the frequency of the swallowing reflex. Experiments were carried out on 85 male Sprague-Dawley rats. The effects of short and sustained application of chemicals (3 µl of 0.1 N HCl or capsaicin) on the frequency of swallowing and of time-dependent changes in the occurrence of swallowing evoked by supralaryngeal nerve stimulation were determined. To evaluate vascular permeability of the larynx, Evans blue dye was intravenously injected after 5 or 60 min of sustained TRPV1 activation. SB366791 (a TRPV1 inhibitor) and Cap/QX-314 (a TRPV1-expressed neuronal inhibitor) significantly inhibited HCl/capsaicin-induced swallowing, but air flow-induced swallowing was not affected. Although the number of air flow-induced swallows induced by capsaicin stimulation was not affected within 5 min, but significantly reduced by 60-min capsaicin or HCl application. The swallowing threshold associated with supralaryngeal nerve stimulation did not significantly change throughout the recording period. Evans blue dye concentrations in the larynx were significantly higher at 60 min in the 10-5 M capsaicin group than in the other groups. Our results suggest that sustained TPRV1 activation not only desensitizes TRPV1, but also inactivates mechanoreceptors, which may be attributed to increases in vascular permeability and edema, as part of an inflammatory process.

リンク情報
DOI
https://doi.org/10.1152/ajpgi.00082.2020
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32755305

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