論文

査読有り
2017年3月

Phloridzin inhibits high K+-induced contraction via the inhibition of sodium: glucose cotransporter 1 in rat ileum

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Hidenori Kanda
  • ,
  • Takeharu Kaneda
  • ,
  • Akira Kawaguchi
  • ,
  • Noriyasu Sasaki
  • ,
  • Tsuyoshi Tajima
  • ,
  • Norimoto Urakawa
  • ,
  • Kazumasa Shimizu
  • ,
  • Hiroetsu Suzuki

79
3
開始ページ
593
終了ページ
601
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.16-0560
出版者・発行元
JAPAN SOC VET SCI

Recent studies have shown that phloridzin, an inhibitor of sodium glucose cotransporter (SGLT), strongly decreases high K+-induced contraction in phasic muscle, such as tenia coil, but slightly affects tonic muscle, such as trachea. In this study, we examined the inhibitory mechanism of phloridzin on high K+-induced muscle contraction in rat ileum, a phasic muscle. Phloridzin inhibited the high K+-induced contraction in the ileum and the aorta, and the relaxing effect of phloridzin at 1 mM in the ileum was approximately five-fold more potent than that in the aorta. The expression of SGLT1 mRNA in the ileum was higher than that of the aorta. Phloridzin significantly inhibited NADH/NAD ratio and phosphocreatine (PCr) content in the ileum; I however, application of pyruvate recovered the inhibition of contraction and PCr content, but had no effect on ratio of NADH/NAD. High K+ increased 2-(N (7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) uptake in ileal smooth muscle cells, and phloridzin inhibited the increase in a concentration-dependent manner. These results suggest that phloridzin inhibits high K+-induced contraction because of the inhibition of energy metabolism via the inhibition of SGLT1.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1292/jvms.16-0560
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28190822
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397830400024&DestApp=WOS_CPL