2007年8月
Purification and characterization of a novel alcohol oxidase from Paenibacillus sp AIU 311
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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- 巻
- 104
- 号
- 2
- 開始ページ
- 124
- 終了ページ
- 128
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1263/jbb.104.124
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
An oxidase catalyzing the conversion of glycolaldehyde to glyoxal was purified to the homogeneous state from Paenibacillus sp. AIU 311, and its properties were revealed. This enzyme was specific to glycolaldehyde and glyceraldehyde, and the reaction rates to other alcohols and aldehydes were less than 6% of that of glycolaldehyde. The K. values for glycolaldehyde and glyceraldehyde were estimated to be 13.2 and 7.5 mM, respectively. The glycolaldehyde oxidation was optimum at pH 6.5 and 50 degrees C. The molecular mass of this enzyme was 49 kDa, and it consisted of two identical subunits of 24 kDa. The NH2-terminal sequence was not homologous to those of alcohol oxidases. This is the first report of an oxidase exhibiting high specificity to a hydroxy group of aldehyde alcohols.
- リンク情報
- ID情報
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- DOI : 10.1263/jbb.104.124
- ISSN : 1389-1723
- eISSN : 1347-4421
- CiNii Articles ID : 110006380959
- Web of Science ID : WOS:000250213000006