論文

査読有り
2007年11月

The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Takanori Kobayashi
  • ,
  • Yuko Ogo
  • ,
  • Reiko Nakanishi Itai
  • ,
  • Hiromi Nakanishi
  • ,
  • Michiko Takahashi
  • ,
  • Satoshi Mori
  • ,
  • Naoko K. Nishizawa

104
48
開始ページ
19150
終了ページ
19155
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.0707010104
出版者・発行元
NATL ACAD SCIENCES

iron is essential for most living organisms and is often the major limiting nutrient for normal growth. Plants induce iron utilization systems under conditions of low iron availability, but the molecular mechanisms of gene regulation under iron deficiency remain largely unknown. We identified the rice transcription factor IDEF1, which specifically binds the iron deficiency-responsive cis-acting element IDE1. IDEF1 belongs to an uncharacterized branch of the plant-specific transcription factor family AB13/VP1 and exhibits the sequence recognition property of efficiently binding to the CATGC sequence within IDE1. IDEF1 transcripts are constitutively present in rice roots and leaves. Transgenic tobacco plants expressing IDEF1 under the control of the constitutive cauliflower mosaic virus 35S promoter transactivate IDE1-mediated expression only in iron-deficient roots. Transgenic rice plants expressing an introduced IDEF1 exhibit substantial tolerance to iron deficiency in both hydroponic culture and calcareous soil. IDEF1 overexpression leads to the enhanced expression of the iron deficiency-induced transcription factor gene Os/RO2, suggesting the presence of a sequential gene regulatory network. These findings reveal cis element/trans factor interactions that are functionally linked to the iron deficiency response. Manipulation of IDEF1 also provides another approach for producing crops tolerant of iron deficiency to enhance food and biomass production in calcareous soils.

リンク情報
DOI
https://doi.org/10.1073/pnas.0707010104
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000251498700052&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.0707010104
  • ISSN : 0027-8424
  • Web of Science ID : WOS:000251498700052

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