2005年6月
Isolation and physiochemical properties of protein-rich nematode mitochondrial ribosomes
Biochemistry
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- 巻
- 44
- 号
- 25
- 開始ページ
- 9232
- 終了ページ
- 9237
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/bi047833c
- 出版者・発行元
- AMER CHEMICAL SOC
In the present study, mitochondrial ribosomes of the nematode Ascaris suum were isolated and their physiochernical properties were compared to ribosomes of Escherichia coli. The sedimentation coefficient and buoyant density of A. suum mitochondrial ribosomes were determined. The sedimentation coefficient of the intact monosome was about 55 S. The buoyant density of formaldehyde-fixed ribosomes in cesium chloride was 1.40 g/cm(3), which suggests that the nematode mitoribosomes have a much higher protein composition than other mitoribosomes. The diffusion coefficients obtained from dynamic light scattering measurements were (1.48 +/- 0.04) x 10(-7) cm(2) s(-1) for 55 S mitoribosomes and (1.74 +/- 0.04) X 10(-7) cm(2) S-1 for the 70 S E. coli monosome. The diameter of mitoribosomes was measured by dynamic light-scattering analysis and electron microscopy. Though the nematode mitoribosome has a larger size than the bacterial ribosome, it does not differ significantly in size from mammalian mitoribosomes.
- リンク情報
- ID情報
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- DOI : 10.1021/bi047833c
- ISSN : 0006-2960
- Web of Science ID : WOS:000230063300031