2008年10月
Isolation of small RNAs using biotinylated PNAs
Journal of Biochemistry
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- 巻
- 144
- 号
- 4
- 開始ページ
- 415
- 終了ページ
- 418
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/jb/mvn107
- 出版者・発行元
- OXFORD UNIV PRESS
In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.
- リンク情報
- ID情報
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- DOI : 10.1093/jb/mvn107
- ISSN : 0021-924X
- Web of Science ID : WOS:000259770200001