論文

査読有り 招待有り 筆頭著者 国際誌
2021年6月

Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses

STAR Protocols
  • Nobuyuki Okahashi
  • ,
  • Masahiro Ueda
  • ,
  • Shu Yasuda
  • ,
  • Hiroshi Tsugawa
  • ,
  • Makoto Arita

2
2
開始ページ
100492
終了ページ
100492
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.xpro.2021.100492
出版者・発行元
Elsevier {BV}

We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 program provides information on known and unknown complex lipid molecules. The FBMN technique clusters similar MS2 spectra, facilitating the identification of bacterial lipids. Targeted analysis was used as a complementary method to cover oxylipins. Here, we provide details for targeted and untargeted analyses. For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2020).

リンク情報
DOI
https://doi.org/10.1016/j.xpro.2021.100492
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33997812
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091925
ID情報
  • DOI : 10.1016/j.xpro.2021.100492
  • ISSN : 2666-1667
  • ORCIDのPut Code : 93446406
  • PubMed ID : 33997812
  • PubMed Central 記事ID : PMC8091925

エクスポート
BibTeX RIS