2021年6月
Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses
STAR Protocols
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- 巻
- 2
- 号
- 2
- 開始ページ
- 100492
- 終了ページ
- 100492
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.xpro.2021.100492
- 出版者・発行元
- Elsevier {BV}
We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 program provides information on known and unknown complex lipid molecules. The FBMN technique clusters similar MS2 spectra, facilitating the identification of bacterial lipids. Targeted analysis was used as a complementary method to cover oxylipins. Here, we provide details for targeted and untargeted analyses. For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2020).
- リンク情報
- ID情報
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- DOI : 10.1016/j.xpro.2021.100492
- ISSN : 2666-1667
- ORCIDのPut Code : 93446406
- PubMed ID : 33997812
- PubMed Central 記事ID : PMC8091925