論文

査読有り 筆頭著者 責任著者
2015年11月

A comprehensive strategy for identifying long-distance mobile peptides in xylem sap

PLANT JOURNAL
  • Satoru Okamoto
  • ,
  • Takamasa Suzuki
  • ,
  • Masayoshi Kawaguchi
  • ,
  • Tetsuya Higashiyama
  • ,
  • Yoshikatsu Matsubayashi

84
3
開始ページ
611
終了ページ
620
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/tpj.13015
出版者・発行元
WILEY

There is a growing awareness that secreted peptides mediate organ-to-organ communication in higher plants. Xylem sap peptidomics is an effective but challenging approach for identifying long-distance mobile peptides. In this study we developed a simple, gel-free purification system that combines o-chlorophenol extraction with HPLC separation. Using this system, we successfully identified seven oligopeptides from soybean xylem sap exudate that had one or more post-transcriptional modifications: glycosylation, sulfation and/or hydroxylation. RNA sequencing and quantitative PCR analyses showed that the peptide-encoding genes are expressed in multiple tissues. We further analyzed the long-distance translocation of four of the seven peptides using gene-encoding peptides with single amino acid substitutions, and identified these four peptides as potential root-to-shoot mobile oligopeptides. Promoter-GUS analysis showed that all four peptide-encoding genes were expressed in the inner tissues of the root endodermis. Moreover, we found that some of these peptide-encoding genes responded to biotic and/or abiotic factors. These results indicate that our purification system provides a comprehensive approach for effectively identifying endogenous small peptides and reinforce the concept that higher plants employ various peptides in root-to-shoot signaling.

リンク情報
DOI
https://doi.org/10.1111/tpj.13015
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26333921
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365410300013&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/tpj.13015
  • ISSN : 0960-7412
  • eISSN : 1365-313X
  • PubMed ID : 26333921
  • Web of Science ID : WOS:000365410300013

エクスポート
BibTeX RIS