2015年11月
A comprehensive strategy for identifying long-distance mobile peptides in xylem sap
PLANT JOURNAL
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- 巻
- 84
- 号
- 3
- 開始ページ
- 611
- 終了ページ
- 620
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/tpj.13015
- 出版者・発行元
- WILEY
There is a growing awareness that secreted peptides mediate organ-to-organ communication in higher plants. Xylem sap peptidomics is an effective but challenging approach for identifying long-distance mobile peptides. In this study we developed a simple, gel-free purification system that combines o-chlorophenol extraction with HPLC separation. Using this system, we successfully identified seven oligopeptides from soybean xylem sap exudate that had one or more post-transcriptional modifications: glycosylation, sulfation and/or hydroxylation. RNA sequencing and quantitative PCR analyses showed that the peptide-encoding genes are expressed in multiple tissues. We further analyzed the long-distance translocation of four of the seven peptides using gene-encoding peptides with single amino acid substitutions, and identified these four peptides as potential root-to-shoot mobile oligopeptides. Promoter-GUS analysis showed that all four peptide-encoding genes were expressed in the inner tissues of the root endodermis. Moreover, we found that some of these peptide-encoding genes responded to biotic and/or abiotic factors. These results indicate that our purification system provides a comprehensive approach for effectively identifying endogenous small peptides and reinforce the concept that higher plants employ various peptides in root-to-shoot signaling.
- リンク情報
- ID情報
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- DOI : 10.1111/tpj.13015
- ISSN : 0960-7412
- eISSN : 1365-313X
- PubMed ID : 26333921
- Web of Science ID : WOS:000365410300013