論文

査読有り 国際誌
2018年5月

Sensing of substratum rigidity and directional migration by fast-crawling cells.

Physical review. E
  • Chika Okimura
  • ,
  • Yuichi Sakumura
  • ,
  • Katsuya Shimabukuro
  • ,
  • Yoshiaki Iwadate

97
5-1
開始ページ
052401
終了ページ
052401
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1103/PhysRevE.97.052401

Living cells sense the mechanical properties of their surrounding environment and respond accordingly. Crawling cells detect the rigidity of their substratum and migrate in certain directions. They can be classified into two categories: slow-moving and fast-moving cell types. Slow-moving cell types, such as fibroblasts, smooth muscle cells, mesenchymal stem cells, etc., move toward rigid areas on the substratum in response to a rigidity gradient. However, there is not much information on rigidity sensing in fast-moving cell types whose size is ∼10 μm and migration velocity is ∼10 μm/min. In this study, we used both isotropic substrata with different rigidities and an anisotropic substratum that is rigid on the x axis but soft on the y axis to demonstrate rigidity sensing by fast-moving Dictyostelium cells and neutrophil-like differentiated HL-60 cells. Dictyostelium cells exerted larger traction forces on a more rigid isotropic substratum. Dictyostelium cells and HL-60 cells migrated in the "soft" direction on the anisotropic substratum, although myosin II-null Dictyostelium cells migrated in random directions, indicating that rigidity sensing of fast-moving cell types differs from that of slow types and is induced by a myosin II-related process.

リンク情報
DOI
https://doi.org/10.1103/PhysRevE.97.052401
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29906928

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