論文

査読有り 国際誌
2016年7月3日

Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes.

Cell adhesion & migration
  • Chika Okimura
  • ,
  • Yoshiaki Iwadate

10
4
開始ページ
406
終了ページ
18
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/19336918.2016.1170268

Crawling cells can generate polarity for migration in response to forces applied from the substratum. Such reaction varies according to cell type: there are both fast- and slow-crawling cells. In response to periodic stretching of the elastic substratum, the intracellular stress fibers in slow-crawling cells, such as fibroblasts, rearrange themselves perpendicular to the direction of stretching, with the result that the shape of the cells extends in that direction; whereas fast-crawling cells, such as neutrophil-like differentiated HL-60 cells and Dictyostelium cells, which have no stress fibers, migrate perpendicular to the stretching direction. Fish epidermal keratocytes are another type of fast-crawling cell. However, they have stress fibers in the cell body, which gives them a typical slow-crawling cell structure. In response to periodic stretching of the elastic substratum, intact keratocytes rearrange their stress fibers perpendicular to the direction of stretching in the same way as fibroblasts and migrate parallel to the stretching direction, while blebbistatin-treated stress fiber-less keratocytes migrate perpendicular to the stretching direction, in the same way as seen in HL-60 cells and Dictyostelium cells. Our results indicate that keratocytes have a hybrid mechanosensing system that comprises elements of both fast- and slow-crawling cells, to generate the polarity needed for migration.

リンク情報
DOI
https://doi.org/10.1080/19336918.2016.1170268
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27124267
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986709

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