論文

2016年7月

Microbial production of dihomo-γ-linolenic acid by Δ5-desaturase gene-disruptants of Mortierella alpina 1S-4.

Journal of bioscience and bioengineering
  • Hiroshi Kikukawa
  • ,
  • Eiji Sakuradani
  • ,
  • Akinori Ando
  • ,
  • Tomoyo Okuda
  • ,
  • Sakayu Shimizu
  • ,
  • Jun Ogawa

122
1
開始ページ
22
終了ページ
6
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiosc.2015.12.007

We constructed dihomo-γ-linolenic acid (DGLA)-producing strains with disruption of the Δ5-desaturase (Δ5ds) gene, which encodes a key enzyme catalyzing the bioconversion of DGLA to arachidonic acid (ARA), by efficient gene-targeting, using Δlig4 strain of Mortierella alpina 1S-4 as the host. In previous study, we had already identified and disrupted the lig4 gene encoding DNA ligase 4, which involves in non-homologous end joining, in M. alpina 1S-4, and the Δlig4 strain had showed efficient gene-targeting. In this study, the uracil auxotroph of Δlig4 strain was constructed, and then transformed for disruption of Δ5ds. The isolation of nine Δ5ds-disruptants out of 18 isolates indicated that the disruption efficiency was 50%. The ratio of DGLA among the total fatty acids of the Δ5ds-disruptants reached 40.1%; however, no ARA was detected. To our knowledge, this is the first study to report the construction of DGLA-producing transformants by using the efficient gene-targeting system in M. alpina 1S-4.

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2015.12.007
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26777235
ID情報
  • DOI : 10.1016/j.jbiosc.2015.12.007
  • PubMed ID : 26777235

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