論文

2015年9月

Omega-3 eicosatetraenoic acid production by molecular breeding of the mutant strain S14 derived from Mortierella alpina 1S-4.

Journal of bioscience and bioengineering
  • Tomoyo Okuda
  • ,
  • Akinori Ando
  • ,
  • Hiroaki Negoro
  • ,
  • Hiroshi Kikukawa
  • ,
  • Takaiku Sakamoto
  • ,
  • Eiji Sakuradani
  • ,
  • Sakayu Shimizu
  • ,
  • Jun Ogawa

120
3
開始ページ
299
終了ページ
304
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiosc.2015.01.014
出版者・発行元
SOC BIOSCIENCE BIOENGINEERING JAPAN

We investigated the omega-3 eicosatetraenoic acid (ETA) production by molecular breeding of the oleaginous fungus Mortierella alpina, which can slightly accumulate ETA only when cultivated at a low temperature. The endogenous ω3-desaturase gene or the heterologous Saprolegnia diclina Δ17 (sdd17m) desaturase gene were overexpressed in M. alpina S14, a Δ5-desaturation activity-defective mutant derived from M. alpina 1S-4. M. alpina S14 transformants introduced with the endogenous ω3-desaturase gene showed ETA at 42.1% content in the total lipids that was 84.2-fold and 3.2-fold higher than that of the wild-type strain 1S-4 and host strain S14, respectively, when cultivated at 12°C. No accumulation of ETA was observed at 28°C. In contrast, transformants with the heterologous sdd17m gene showed 24.9% of the content of total lipids at 28°C. These results indicated that these M. alpina S14 transformants are promising strains for the production of ETA, which is hard to obtain from natural sources.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2015.01.014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25845716
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000362146400011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jbiosc.2015.01.014
  • ISSN : 1389-1723
  • eISSN : 1347-4421
  • PubMed ID : 25845716
  • Web of Science ID : WOS:000362146400011

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