論文

国際誌
2015年11月

Gene targeting in the oil-producing fungus Mortierella alpina 1S-4 and construction of a strain producing a valuable polyunsaturated fatty acid.

Current genetics
  • Hiroshi Kikukawa
  • ,
  • Eiji Sakuradani
  • ,
  • Masato Nakatani
  • ,
  • Akinori Ando
  • ,
  • Tomoyo Okuda
  • ,
  • Takaiku Sakamoto
  • ,
  • Misa Ochiai
  • ,
  • Sakayu Shimizu
  • ,
  • Jun Ogawa

61
4
開始ページ
579
終了ページ
89
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00294-015-0481-2

To develop an efficient gene-targeting system in Mortierella alpina 1S-4, we identified the ku80 gene encoding the Ku80 protein, which is involved in the nonhomologous end-joining pathway in genomic double-strand break (DSB) repair, and constructed ku80 gene-disrupted strains via single-crossover homologous recombination. The Δku80 strain from M. alpina 1S-4 showed no negative effects on vegetative growth, formation of spores, and fatty acid productivity, and exhibited high sensitivity to methyl methanesulfonate, which causes DSBs. Dihomo-γ-linolenic acid (DGLA)-producing strains were constructed by disruption of the Δ5-desaturase gene, encoding a key enzyme of bioconversion of DGLA to ARA, using the Δku80 strain as a host strain. The significant improvement of gene-targeting efficiency was not observed by disruption of the ku80 gene, but the construction of DGLA-producing strain by disruption of the Δ5-desaturase gene was succeeded using the Δku80 strain as a host strain. This report describes the first study on the identification and disruption of the ku80 gene in zygomycetes and construction of a DGLA-producing transformant using a gene-targeting system in M. alpina 1S-4.

リンク情報
DOI
https://doi.org/10.1007/s00294-015-0481-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25782448
ID情報
  • DOI : 10.1007/s00294-015-0481-2
  • PubMed ID : 25782448

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