論文

国際誌
2014年8月

Characterization of galactose-dependent promoters from an oleaginous fungus Mortierella alpina 1S-4.

Current genetics
  • Tomoyo Okuda
  • ,
  • Akinori Ando
  • ,
  • Eiji Sakuradani
  • ,
  • Hiroshi Kikukawa
  • ,
  • Nozomu Kamada
  • ,
  • Misa Ochiai
  • ,
  • Jun Shima
  • ,
  • Jun Ogawa

60
3
開始ページ
175
終了ページ
82
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00294-014-0422-5

An inducible promoter is a useful tool for the controlled expression of a given gene. In this report, we describe galactose-dependent promoters for potential use in an oleaginous fungus Mortierella alpina. We cloned the putative promoter regions of two genes encoding galactose metabolic enzymes, GAL1 and GAL10, from the genome of M. alpina 1S-4. The β-glucuronidase (GUS) reporter gene assay in M. alpina 1S-4 revealed that regulation of these promoters was dependent on the presence of galactose in the medium both with and without other sugars. With the GAL10 promoter, an approximately 50-fold increase of GUS activity was demonstrated by addition of galactose into the culture media at any cultivation phase. The 5' deletion analysis of the GAL10 promoter revealed that a promoter region of over 2,000 bp length was required for its high-level activity and sufficient inducible response. Significantly, this is the first report of inducible promoters of zygomycetes. The GAL10 promoter will be a valuable tool for gene manipulation in M. alpina 1S-4.

リンク情報
DOI
https://doi.org/10.1007/s00294-014-0422-5
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24549368
ID情報
  • DOI : 10.1007/s00294-014-0422-5
  • PubMed ID : 24549368

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