論文

2015年12月

Eicosapentaenoic acid (EPA) production by an oleaginous fungus Mortierella alpina expressing heterologous the Delta 17-desaturase gene under ordinary temperature

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
  • Tomoyo Okuda
  • ,
  • Akinori Ando
  • ,
  • Hiroaki Negoro
  • ,
  • Tatsuya Muratsubaki
  • ,
  • Hiroshi Kikukawa
  • ,
  • Takaiku Sakamoto
  • ,
  • Eiji Sakuradani
  • ,
  • Sakayu Shimizu
  • ,
  • Jun Ogawa

117
12
開始ページ
1919
終了ページ
1927
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/ejlt.201400657
出版者・発行元
WILEY

The oleaginous fungus Mortierella alpina is known to accumulate eicosapentaenoic acid (EPA) only when cultivated at a low temperature (below 15 degrees C). Here, we investigated EPA production at an ordinary temperature (28 degrees C) by expressing the Saprolegnia diclina Delta 17 desaturase gene (sdd17m) in M. alpina ST1358, an omega 3-desaturation activity-defective mutant derived from M. alpina 1S-4. Expression of the exogenous gene was confirmed by EPA accumulation in transformants at both 28 and 12 degrees C. The EPA content in total lipids produced by transformants was over 20% at 28 degrees C. Bench-scale fermentation with a 5-L jar fermentor showed that EPA content reached 26.4% of total fatty acids, and EPA production reached 1.8 g/L. This is the first study to report the accumulation of EPA in M. alpina at an ordinary temperature, and provide a platform technology for the industrial production of EPA using M. alpina as a promising source for EPA.

Web of Science ® 被引用回数 : 19

リンク情報
DOI
https://doi.org/10.1002/ejlt.201400657
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000367008500003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/ejlt.201400657
  • ISSN : 1438-7697
  • eISSN : 1438-9312
  • Web of Science ID : WOS:000367008500003

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