OMASA Takeshi

Last updated: Dec 5, 2019 at 04:39

Name	OMASA Takeshi
E-mail	omasabio.eng.osaka-u.ac.jp
Affiliation	Osaka University
Section	Graduate School of Engineering Division of Science and Biotechnology
Job title	Professor
Research funding number	00252586
ORCID ID	0000-0003-0635-3862

Research Areas

Process/Chemical engineering / Biofunction/Bioprocess /

Academic & Professional Experience

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May 2010

教授, 徳島大学大学院ソシオテクノサイエンス研究部

Published Papers

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Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production

Kohei Kaneyoshi, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano, Yuichi Koga, and Takeshi Omasa*

Journal of Bioscience and Bioengineering 127(1) 107-113 Jan 2019 [Refereed]

Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and mouse using end sequences of CHO BAC clones based on BAC-FISH results.

Shuichi Kimura, and Takeshi Omasa*

Cytotechnology 70(5) 1399-1407 Oct 2018 [Refereed]

The healing effect of human milk fat globule-EGF factor 8 protein (MFG-E8) in a rat model of Parkinson’s disease

Yoshiki Nakashima*, Chika Miyagi-Shiohira, Hirofumi Noguchi and Takeshi Omasa

Brain Science 8(9) 167 Sep 2018 [Refereed]

Atorvastatin inhibits the HIF1α-PPAR axis, which is essential for maintaining the function of human induced pluripotent stem cells

Yoshiki Nakashima*, Chika Miyagi-Shiohira, Hirofumi Noguchi, and Takeshi Omasa

Molecular Therapy 26(7) 1715-1734 Aug 2018 [Refereed]

Development of hyper osmotic resistant CHO host cells for enhanced antibody production.

Yasuharu Kamachi*, and Takeshi Omasa

Journal of Bioscience and Bioengineering 125(4) 470-478 Aug 2018 [Refereed]

Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells.

Masayoshi Onitsuka*, Yukie Kinoshita, Akitoshi Nishizawa, Tomomi Tsutsui and Takeshi Omasa

Cytotechnology 70(2) 675-685 Apr 2018 [Refereed]

The enhancement of antibody concentration and achievement of high cell density CHO cell cultivation by adding nucleoside

Yasuhiro Takagi, Takuya Kikuchi, Ryuta Wada and Takeshi Omasa*

Cytotechnology 69(3) 511-521 Jun 2017 [Refereed]

Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site

Yoshinori Kawabe, Takanori Inao, Shodai Komatsu, Guan Huang, Akira Ito, Takeshi Omasa, and Masamichi Kamihira

Journal of Bioscience and Bioengineering 123(3) 390-397 Mar 2017 [Refereed]

Lengthening of high-yield production levels of monoclonal antibody-producing Chinese hamster ovary cells by downregulation of breast cancer 1

Rima Matsuyama¶, Noriko Yamano¶, Namiko Kawamura, and Takeshi Omasa*(¶:equal contribution)

Journal of Bioscience and Bioengineering 123(3) 382-389 Mar 2017 [Refereed]

Identification of regulatory motifs in the CHO genome for stable monoclonal antibody production

Yasuhiro Takagi, Tomomi Yamazaki, Kenji Masuda, Shigeaki Nishii, Bunsei Kawakami and Takeshi Omasa*

Cytotechnology 69(3) 451-460 Jun 2016 [Refereed]

Increased recombinant protein production owing to expanded opportunities for vector integration in high chromosome number Chinese hamster ovary cells

Noriko Yamano, Mai Takahashi, Seyed Mohammad Ali Haghparast, Masayoshi Onitsuka, Toshitaka Kumamoto, Jana Frank, and Takeshi Omasa

Journal of Bioscience and Bioengineering 122(2) 226-231 Feb 2016 [Refereed]

Rapid colorimetric antibody detection using a dual-function peptide probe for silver nanoparticle aggregation and antibody recognition

Mina Okochi*, Tomohiro Kamiya, Takeshi Omasa, Masayoshi Tanaka, Hiroyuki Honda

Analytical Sciences 32(1) 93-97 Jan 2016 [Refereed]

Metabolic analysis of antibody producing CHO cell culture under different stresses conditions

Md. Bahadur Badsha, Hiroyuki Kurata*, Masayoshi Onitsuka, Takushi Oga, Takeshi Omasa

Journal of Bioscience and Bioengineering 122(1) 117-124 Jan 2016 [Refereed]

Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells

Rima Matsuyama, Tomomi Tsutsui, Kyoung Ho Lee, Masayoshi Onitsuka, and Takeshi Omasa

Journal of Bioscience and Bioengineering 120(6) 701-708 Jun 2015 [Refereed]

Approaches to quality risk management when using single use systems in the manufacture of biologics

Akiko Ishii-Watabe*, Akihiko Hirose, Noriko Katori, Norikata Hashii, Susumu Arai, Hirotoshi Awatsu, Akira Eiza, Yoshiaki Hara, Hideshi Hattori, Tomomi Inoue, Tetsuya Isono, Masahiro Iwakura, Daisuke Kajihara, Nobuo Kasahara, Hiroyuki Matsuda, Sei Murakami, Taishiro Nakagawa, Takehiro Okumura, Takeshi Omasa, Shinya Takuma, Iyo Terashima, Masayoshi Tsukahara, Maiko Tsutsui, Takahiro Yano, Nana Kawasaki

AAPS PharmSciTech 16(5) 993-1001 May 2015 [Refereed]

Optimization of cell line development in the GS-CHO expression system using a high-throughput, single cell-based clone selection system.

Nakamura T, Omasa T

Journal of bioscience and bioengineering Mar 2015 [Refereed]

Efficient enrichment of high-producing recombinant Chinese hamster ovary cells for monoclonal antibody by flow cytometry.

Okumura T, Masuda K, Watanabe K, Miyadai K, Nonaka K, Yabuta M, Omasa T

Journal of bioscience and bioengineering Feb 2015 [Refereed]

Effect of polyphenols on reactive oxygen species production and cell growth of human dermal fibroblasts after irradiation with ultraviolet-a light.

Shirai A, Onitsuka M, Maseda H, Omasa T

Biocontrol science 20 27-33 2015 [Refereed]

Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay.

Onitsuka M, Omasa T

Journal of bioscience and bioengineering Dec 2014 [Refereed]

Neuraminidase gene homology contributes to the protective activity of influenza vaccines prepared from the influenza virus library.

Haredy AM, Yamada H, Sakoda Y, Okamatsu M, Yamamoto N, Omasa T, Mori Y, Kida H, Okamoto S, Okuno Y, Yamanishi K

The Journal of general virology 95 2365-2371 Nov 2014 [Refereed]

The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad.

Kishimoto N, Momota Y, Hashimoto Y, Tatsumi S, Ando K, Omasa T, Kotani J

Clinical oral investigations 18 1893-1901 Nov 2014 [Refereed]

Rearranging the domain order of a diabody-based IgG-like bispecific antibody enhances its antitumor activity and improves its degradation resistance and pharmacokinetics.

Asano R, Shimomura I, Konno S, Ito A, Masakari Y, Orimo R, Taki S, Arai K, Ogata H, Okada M, Furumoto S, Onitsuka M, Omasa T, Hayashi H, Katayose Y, Unno M, Kudo T, Umetsu M, Kumagai I

mAbs 6 1243-1254 2014 [Refereed]

Improved antibody production in Chinese hamster ovary cells by ATF4 overexpression.

Haredy AM, Nishizawa A, Honda K, Ohya T, Ohtake H, Omasa T

Cytotechnology Sep 2013 [Refereed]

An MDCK cell culture-derived formalin-inactivated influenza virus whole-virion vaccine from an influenza virus library confers cross-protective immunity by intranasal administration in mice.

Haredy AM, Takenaka N, Yamada H, Sakoda Y, Okamatsu M, Yamamoto N, Omasa T, Ohtake H, Mori Y, Kida H, Yamanishi K, Okamoto S

Clinical and vaccine immunology : CVI 20 998-1007 Jul 2013 [Refereed]

Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering.

Lee KH, Onitsuka M, Honda K, Ohtake H, Omasa T

Applied microbiology and biotechnology 97 5731-5741 Jul 2013 [Refereed]

Generation of high-producing cell lines by overexpression of cell division cycle 25 homolog A in Chinese hamster ovary cells.

Lee KH, Tsutsui T, Honda K, Asano R, Kumagai I, Ohtake H, Omasa T

Journal of bioscience and bioengineering Jun 2013 [Refereed]

Two routes of MexS-MexT-mediated regulation of MexEF-OprN and MexAB-OprM efflux pump expression in Pseudomonas aeruginosa.

Uwate M, Ichise YK, Shirai A, Omasa T, Nakae T, Maseda H

Microbiology and immunology 57 263-272 Apr 2013 [Refereed]

Development of a continuous bioconversion system using a thermophilic whole-cell biocatalyst.

Ninh PH, Honda K, Yokohigashi Y, Okano K, Omasa T, Ohtake H

Applied and environmental microbiology 79 1996-2001 Mar 2013 [Refereed]

Synthesis and biological activity of thiazolyl-acetic acid derivatives as possible antimicrobial agents.

Shirai A, Fumoto Y, Shouno T, Maseda H, Omasa T

Biocontrol science 18 59-73 2013 [Refereed]

Synthesis of thiazole derivatives and evaluation of their antiamoebic activity and cytotoxicity.

Shirai A, Endo T, Maseda H, Omasa T

Biocontrol science 18 183-191 2013 [Refereed]

Inhibition of CYP3A4 by 6',7'-dihydroxybergamottin in human CYP3A4 over-expressed hepG2 cells.

Araki N, Tsuruoka S, Hasegawa G, Yanagihara H, Omasa T, Enosawa S, Yamazoe Y, Fujimura A

The Journal of pharmacy and pharmacology 64 1715-1721 Dec 2012 [Refereed]

Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway.

Ye X, Honda K, Sakai T, Okano K, Omasa T, Hirota R, Kuroda A, Ohtake H

Microbial cell factories 11 120 Sep 2012 [Refereed]

Identification of the replication region of a 111-kb circular plasmid from Rhodococcus opacus B-4 by λ Red recombination-based deletion analysis.

Honda K, Imura M, Okano K, Omasa T, Kato J, Ohtake H

Bioscience, biotechnology, and biochemistry 76 1758-1764 2012 [Refereed]

Chromosome identification and its application in Chinese hamster ovary cells.

Cao Y, Kimura S, Park JY, Yamatani M, Honda K, Ohtake H, Omasa T

BMC proceedings 5 Suppl 8 O8 2011 [Refereed]

ATF4 over-expression increased IgG1 productivity in Chinese hamster ovary cells.

Haredy AM, Nishizawa A, Honda K, Ohya T, Ohtake H, Omasa T

BMC proceedings 5 Suppl 8 O11 2011 [Refereed]

Identification and analysis of specific chromosomal region adjacent to exogenous Dhfr-amplified region in Chinese hamster ovary cell genome.

Park JY, Takagi Y, Yamatani M, Honda K, Asakawa S, Shimizu N, Omasa T, Ohtake H

Journal of bioscience and bioengineering 109(5) 504-511 May 2010 [Refereed]

Electron Microscopic Analysis of Heat-Induced Leakage of Polyphosphate from a phoU Mutant of Escherichia coli.

Kashihara H, Kang BM, Omasa T, Honda K, Sameshima Y, Kuroda A, Ohtake H

Bioscience, biotechnology, and biochemistry 74(4) 865-868 Apr 2010 [Refereed]

Cell engineering and cultivation of chinese hamster ovary (CHO) cells.

Omasa T, Onitsuka M, Kim WD

Current pharmaceutical biotechnology 11 233-240 Apr 2010 [Refereed]

Systematic screening of Escherichia coli single-gene knockout mutants for improving recombinant whole-cell biocatalysts.

Zhou Y, Minami T, Honda K, Omasa T, Ohtake H

Applied microbiology and biotechnology 87(2) 647-655 Mar 2010 [Refereed]

Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells.

Kim WD, Tokunaga M, Ozaki H, Ishibashi T, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Omasa T, Ohtake H

Applied microbiology and biotechnology 85(3) 535-542 Jan 2010 [Refereed]

Enhanced antibody production following intermediate addition based on flux analysis in mammalian cell continuous culture.

Omasa T, Furuichi K, Iemura T, Katakura Y, Kishimoto M, Suga K

Bioprocess and biosystems engineering 33(1) 117-125 Jan 2010 [Refereed]

Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells.

Omasa T, Cao Y, Park JY, Takagi Y, Kimura S, Yano H, Honda K, Asakawa S, Shimizu N, Ohtake H

Biotechnology and bioengineering 104(5) 986-994 Dec 2009 [Refereed]

Effect of cell-surface hydrophobicity on bacterial conversion of water-immiscible chemicals in two-liquid-phase culture systems.

Hamada T, Maeda Y, Matsuda H, Sameshima Y, Honda K, Omasa T, Kato J, Ohtake H

Journal of bioscience and bioengineering 108(2) 116-120 Aug 2009 [Refereed]

Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells.

Fujiyama K, Misaki R, Sakai Y, Omasa T, Seki T

Journal of bioscience and bioengineering 107(2) 165-172 Feb 2009 [Refereed]

Overexpression of GADD34 enhances production of recombinant human antithrombin III in Chinese hamster ovary cells.

Omasa T, Takami T, Ohya T, Kiyama E, Hayashi T, Nishii H, Miki H, Kobayashi K, Honda K, Ohtake H

Journal of bioscience and bioengineering 106(6) 568-573 Dec 2008 [Refereed]

Atorvastatin inhibits the HIF1α-PPAR axis, which is essential for maintaining the function of human induced pluripotent stem cells

Yoshiki Nakashima, Chika Miyagi-Shiohira, Hirofumi Noguchi, Takeshi Omasa

Molecular Therapy 26(7) 1715-1734 Jul 2018 [Refereed]

Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and mouse using end sequences of CHO BAC clones based on BAC-FISH results

Shuichi Kimura, Takeshi Omasa

Cytotechnology 70(5) 1399-1407 Oct 2018 [Refereed]

The healing effect of human milk fat globule-EGF factor 8 protein (MFG-E8) in a rat model of Parkinson's disease

Yoshiki Nakashima, Chika Miyagi-Shiohira, Hirofumi Noguchi, Takeshi Omasa

Brain Science 8(9) 167 Oct 2018 [Refereed]

Association Memberships

THE SOCIETY FOR BIOTECHNOLOGY, JAPAN(324) , THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN(503) , JAPANESE ASSOCIATION FOR ANIMAL CELL TECHNOLOGY(43) , JAPAN SOCIETY FOR BIOSICENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY(96) , THE JAPANESE SOCIETY FOR REGENERTIVE MEDICINE(JSRM)(28)

Research Grants & Projects

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Construction of high-expression vector had on gene-amplified chromosomal location and its application to human cell

Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))

Project Year: 2005 - 2007 Investigator(s): Takeshi OMASA

Gene amplification means "the selective, repeated replication of a certain gene or genes without a proportional increase in other genes in the genome". Gene amplification is a widespread phenomenon in eukaryotes. It is an important process in the ...