2013年7月21日
Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation inSynechocystissp PCC 6803
Channels
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- 巻
- 7
- 号
- 4
- 開始ページ
- 238
- 終了ページ
- 242
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.4161/chan.25350
- 出版者・発行元
- Informa UK Limited
Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that symscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.
- リンク情報
- ID情報
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- DOI : 10.4161/chan.25350
- ISSN : 1933-6950
- eISSN : 1933-6969
- Web of Science ID : WOS:000327511900003