論文

査読有り 最終著者 責任著者 国際誌
2020年

Hematogenous apoptotic mechanism in salivary glands in chronic periodontitis

Archives of Oral Biology
  • Shikayama, T.
  • Fujita-Yoshigaki, J.
  • Sago-Ito, M.
  • Nakamura-Kiyama, M.
  • Naniwa, M.
  • Hitomi, S.
  • Ujihara, I.
  • Kataoka, S.
  • Yada, N.
  • Ariyoshi, W.
  • Usui, M.
  • Nakashima, K.
  • Ono, K.
  • 全て表示

117
開始ページ
104775
終了ページ
104775
記述言語
日本語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.archoralbio.2020.104775
出版者・発行元
Elsevier BV

OBJECTIVE: The aim of the study is to investigate the apoptotic mechanism in salivary glands in the rat experimental periodontitis model. DESIGN: A rat periodontitis model was prepared by using a ligature around the second upper molar. In the salivary (parotid and submandibular) glands and blood samples, putative apoptotic factors and pathway molecules were investigated in vivo and in vitro. RESULTS: Four weeks of ligation (chronic periodontitis) demonstrated significant apoptotic atrophy of the salivary gland, but one week of ligation (initial periodontitis) did not. In the blood plasma, tumor necrosis factor-α (TNF-α) was increased in the periodontitis model, but interleukin-1β and -6 were not. TNF-α receptor type 1, which has an intracellular apoptotic pathway, was expressed in the salivary glands of rats. Western blot analysis of cultured rat primary salivary gland cells demonstrated that TNF-α induced cleavage of poly (ADP-ribose) polymerase (PARP) and caspase-3 in a dose-dependent manner, indicating apoptosis induction. Additionally, we found increment of circulating lymphocytes in the model. Expression of mRNA and immunoreactive cells for the B lymphocyte marker CD19 were increased in the salivary gland in the model. Western blotting showed that coculture with extracted B cells from the periodontitis model increased cleaved PARP in salivary gland cells. CONCLUSIONS: Chronic periodontitis status leads to an increase in circulating TNF-α and B lymphocyte infiltration, resulting in apoptotic atrophy of the salivary gland as a periodontitis-induced systemic response.

リンク情報
DOI
https://doi.org/10.1016/j.archoralbio.2020.104775
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32512258
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-85085897844&partnerID=MN8TOARS
ID情報
  • DOI : 10.1016/j.archoralbio.2020.104775
  • ISSN : 1879-1506
  • ISSN : 0003-9969
  • ORCIDのPut Code : 97969524
  • PubMed ID : 32512258
  • SCOPUS ID : 85085897844

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