Yuji Hirao

J-GLOBAL         Last updated: Aug 30, 2017 at 19:32
Yuji Hirao
National Agriculture and Food Research Organization
Embryo Production Research Unit, Division of Animal Breeding and Reproduction Research
Job title
Unit Leader

Research Areas



Apr 1990
Mar 1993
Doctoral Course, The Graduate School of Science and Technology, Kobe University

Awards & Honors

May 2017
Complete in vitro generation of fertile oocytes from mouse primordial germ cells, Cozzarelli Prize, The National Academy of Sciences of the United States of America
Winner: Kanako Morohaku, Ren Tanimoto, Keisuke Sasaki, Ryouka Kawahara-Miki, Tomohiro Kono, Katsuhiko Hayashi, Yuji Hirao, Yayoi Obata
Sep 2011
Factors Affecting the Developmental Competence of Bovine and Porcine oocytes Grown In Vitro, The SRD Outstanding Research Award, Society for Reproduction and Development (SRD)
Mar 2015
Conditions affecting growth and developmental competence of mammalian oocytes in vitro, Excellent Paper Award, Animal Science Journal
Sep 2012
Optimization of Oxygen Concentration for Growing Bovine Oocytes In Vitro: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes, JRD Outstanding Paper Awards in 2012, The Journal of Reproduction and Development (JRD)
Winner: Yuj Hirao, Manabu Shimizu, Kosuke Iga, Naoki Takenouchi

Published Papers

Hayashi K, Hikabe O, Obata Y, Hirao Y
Nature protocols   12(9) 1733-1744   Sep 2017   [Refereed]
Morohaku K, Hirao Y, Obata Y
Nature protocols   12(9) 1817-1829   Sep 2017   [Refereed]
Morohaku K, Hirao Y, Obata Y
Annals of biomedical engineering   45(7) 1608-1619   Jul 2017   [Refereed]
Yuji Hirao
Journal of Mammalian Ova Research   34 23-29   Apr 2017
© 2017 Japan Society for Ova Research. The regulation of mammalian oogenesis in vivo is complicated because of numerous constantly changing events caused by ovarian cells interacting with or influencing each other. One of the most intractable ques...
Somfai T, Hirao Y
Methods in molecular biology (Clifton, N.J.)   1524 255-264   2017   [Refereed]
Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K
Nature   539(7628) 299-303   Nov 2016   [Refereed]
Morohaku K, Hirao Y, Obata Y
The Journal of reproduction and development   62(1) 1-5   Feb 2016   [Refereed]
Morohaku K, Tanimoto R, Sasaki K, Kawahara-Miki R, Kono T, Hayashi K, Hirao Y, Obata Y
Proceedings of the National Academy of Sciences of the United States of America   113(32) 9021-9026   Aug 2016   [Refereed]
日本はい移植学雑誌   36(2) 103-107   May 2014
Hirao Y, Somfai T, Naruse K
The Journal of reproduction and development   60(1) 68-72   Mar 2014   [Refereed]
Yuji Hirao, Takashi Miyano
Journal of Mammalian Ova Research   31 79-85   Jan 2014
©2014 Japan Society for Ova Research Mammalian ovaries contain a large number of oocytes, most of which degenerate either before or during the various stages of growth. To utilize these potential female gametes stored in the ovary, it is essential...
Hirao Y, Naruse K, Kaneda M, Somfai T, Iga K, Shimizu M, Akagi S, Cao F, Kono T, Nagai T, Takenouchi N
Biology of reproduction   89(3) 57   Sep 2013   [Refereed]
Hirao Y, Shimizu M, Iga K, Takenouchi N
The Journal of reproduction and development   58(2) 204-211   Apr 2012   [Refereed]
Taketsuru H, Hirao Y, Takenouchi N, Iga K, Miyano T
Zygote (Cambridge, England)   20(4) 407-415   Nov 2012   [Refereed]
Naruse K, Iga K, Shimizu M, Takenouchi N, Akagi S, Somfai T, Hirao Y
The Journal of reproduction and development   58(4) 476-483   Aug 2012   [Refereed]
Hirao Y
The Journal of reproduction and development   58(2) 167-174   Apr 2012   [Refereed]
Yuji Hirao
Reproductive Medicine and Biology   11 1-9   Jan 2012
Various factors such as gonadotrophins, growth factors, and steroid hormones play important roles in the regulation of oocyte/follicular growth in mammalian ovaries. In addition to these factors, there is a bidirectional interaction between oocyte...
Hirao Y
Animal science journal = Nihon chikusan Gakkaiho   82(2) 187-197   Apr 2011   [Refereed]
Yuji Hirao, Manabu Shimizu, Kosuke Iga, Naoki Takenouchi
Journal of Mammalian Ova Research   25 266-271   Jan 2008
The growing oocytes that are present in bovine ovaries in large numbers can be a potential resource for future livestock production. Here we describe a culture system that allows growing oocytes to survive for an extremely long period. Preantral f...
Yuji Hirao, Takashi Miyano
Journal of Mammalian Ova Research   25 56-62   Jan 2008
It has been established that mouse oocytes grow under appropriate culture conditions. To be able to utilize a broad range of growing oocytes, a way of estimating the optimal culture period is required. In the present study, a detailed analysis was...
Hirao Y, Shimizu M, Iga K, Takenouchi N
The Journal of reproduction and development   55(1) 88-93   Feb 2009   [Refereed]
Somfai T, Hirao Y
Methods in molecular biology (Clifton, N.J.)   761 211-225   2011   [Refereed]
HAYASAKA Shunya, CHIBA Kazuyoshi, TAKADA Naokazu, OTSUBO Toshitake, SHIMIZU Manabu, IGA Kosuke, HIRAO Yuji, TAKENOUCHI Naoki
日本胚移植学雑誌 = Japanese journal of embryo transfer   28(3) 94-101   Sep 2006
Yuji Hirao, Naoki Takenouchi, Manabu Shimizu, Kosuke Iga, Takashi Miyano, Takashi Nagai, Takashi Nagai
Journal of Mammalian Ova Research   21 110-117   Jan 2004
In porcine growing oocytes, chromatin remains diffuse. As the oocytes approach their final size, 120 μm, the chromatin becomes partly condensed and forms a perinucleolar sheath. These changes occur simultaneously with a decrease in transcriptional...
Hirao Y, Itoh T, Shimizu M, Iga K, Aoyagi K, Kobayashi M, Kacchi M, Hoshi H, Takenouchi N
Biology of reproduction   70(1) 83-91   Jan 2004   [Refereed]
Hirao Y, Nishimoto N, Kure-bayashi S, Takenouchi N, Yamauchi N, Masuda H, Nagai T
Zygote (Cambridge, England)   11(3) 191-197   Aug 2003   [Refereed]
Senbon S, Hirao Y, Miyano T
The Journal of reproduction and development   49(4) 259-269   Aug 2003   [Refereed]
Bing Y, Che L, Hirao Y, Takenouchi N, Rodríguez-Martínez H, Nagai T
The Journal of reproduction and development   49(2) 159-166   Apr 2003   [Refereed]
Y. Z. Bing, Y. Hirao, N. Takenouchi, L. M. Che, H. Nakamura, J. Yodoi, T. Nagai
Theriogenology   59 863-873   Feb 2003
Thioredoxin (TRX) is an ubiquitous protein disulfide reductase, which is known to be involved in the implantation development of mouse embryos. In the present study, recombinant human TRX was used to evaluate its effect on the promotion of preimpl...
Takashi Miyano, Yuji Hirao
Journal of Mammalian Ova Research   20 78-85   Jan 2003
A huge number of small oocytes are contained in the ovaries of the pig and cow. A small number of them grow from the minimal size of 30 μm in diameter to the final size of 120-125 μm, then mature, and are ovulated. A large number of the remaining ...
Y. Z. Bing, Y. Hirao, K. Iga, L. M. Che, N. Takenouchi, M. Kuwayama, D. Fuchimoto, H. Rodriguez-Martinez, T. Nagai
Reproduction, Fertility and Development   14 125-131   Aug 2002
The present study was conducted to evaluate the effect of cumulus cells on the in vitro maturation (IVM) and glutathione (GSH) synthesis of porcine oocytes cultured in the presence or absence of cysteamine under different oxygen tensions, and on t...
Yuji Hirao, Naoki Takenouchi, Ken ichi Aizawa, Takashi Nagai
Journal of Mammalian Ova Research   19 12-20   Jan 2002
The purpose of this study was to assess the effect of oxygen condition on the survival and growth in vitro of bovine oocyte-cumulus/granulosa cell complexes isolated from early antral follicles. Complexes comprised of a growing oocyte enclosed in ...
Sadanobu Moritake, Yuji Hirao
Journal of Mammalian Ova Research   19 39-45   Jan 2002
Pig early antral follicles (0.5-1.0 mm in diameter) contain oocytes approximately 100 μm in diameter with limited competence for meiotic resumption. Oocyte-cumulus complexes containing parietal granulosa cells (OCGs) were dissected from the ovaria...
Maria M. Viveiros, Yuji Hirao, John J. Eppig
Developmental Biology   235 330-342   Jul 2001
Oocytes from LTXBO mice exhibit a delayed entry into anaphase I and frequently enter interphase after the first meiotic division. This unique oocyte model was used to test the hypothesis that protein kinase C (PKC) may regulate the meiosis I-to-me...
Yinzhong Bing, Limei Che, Yuji Hirao, Naoki Takenouchi, Takashi Nagai, Takashi Nagai
Journal of Reproduction and Development   46 375-379   Dec 2000
This was a study on the developmental ability of porcine embryos reconstructed by microinjection of a nucleus of cultured (non-starving culture) cumulus cells directly into the cytoplasm of oocytes matured and enucleated in vitro. Cumulus-oocyte c...
平尾 雄二, Viveiros Maria M., Eppig John J.
The Journal of Reproduction and Development   46(Suppl.) j65-j70   Dec 2000
John J. Eppig, Karen Wigglesworth, Yuji Hirao
Developmental Biology   224 60-68   Aug 2000
Oocytes of strain LT mice, and related strains such as LTXBO, exhibit a high incidence of arrest in the progression of meiosis at metaphase I (MI) and in spontaneous parthenogenetic activation. Activation of these oocytes within the ovary leads to...
Andras Dinnyés, Yuji Hirao, Takashi Nagai
Cloning and Stem Cells   1 209-216   Dec 1999
The goal of the present research was to study the parthenogenetic activation of porcine oocytes following treatment with the specific cyclin-dependent kinase inhibitor butyrolactone I (BL I). In Experiment I, the effective dose of BL I was determi...
Yuji Hirao, John J. Eppig
Molecular Reproduction and Development   54 311-318   Oct 1999
Fully grown oocytes of most laboratory mice progress without interruption from the germinal vesicle (GV) stage to metaphase II, where meiosis is arrested until fertilization. In contrast, many oocytes of strain LT mice arrest precociously at metap...
Keith E. Latham, F. Dale M Bautista, Yuji Hirao, Marilyn J. O'Brien, John J. Eppig
Biology of Reproduction   61 482-492   Aug 1999
Successful development of mammalian oocytes requires correct interactions between developing oocytes and associated granulosa cells. Development of oocyte-granulosa cell complexes from preantral follicles in vitro does not produce oocytes competen...
宮野 隆, 平尾 雄二
日本胚移植学雑誌   21(1) 16-22   Jan 1999
Yoshi hisa Ikeda, Takashi Miyano, Yuji Hirao
Journal of Mammalian Ova Research   16 148-153   Jan 1999
Ovaries from 4-day-old mice were organ-cultured and the effects of progesterone, androstenedione and estradiol-17β on early follicular development were investigated. Both androstenedione (40‒1,000 ng/ml) and estradiol-17β (8‒1,000 ng/ml) promoted ...
Yuji Hirao, John J. Eppig
Development   124 5107-5113   Dec 1997
Oocytes of almost all vertebrates become arrested at metaphase II to await fertilization. Arrest is achieved with the participation of a protein complex known as cytostatic factor (CSF) that stabilizes histone H1 kinase activity. MOS and mitogen-a...
J. J. Eppig, F. Chesnel, Y. Hirao, M. J. O'Brien, F. L. Pendola, S. Watanabe, K. Wigglesworth
Human reproduction (Oxford, England)   12 127-132   Nov 1997
Interaction between oocytes and granulosa cells is complex and involves both gap junctions and paracrine signalling factors. Oocyte development in antral follicles is highly dependent on communication with cumulus cells, a subset of granulosa cell...
Yuji Hirao, John J. Eppig
Molecular Reproduction and Development   48 391-396   Nov 1997
Ovarian teratomas develop in Mos(-/-) mutant mice produced by homologous recombination. These teratomas are probably derived from oocytes that undergo spontaneous parthenogenetic activation within the ovaries. However, it is not clear how the acti...
John J. Eppig, Karen Wigglesworth, Frank Pendola, Yuji Hirao
Biology of Reproduction   56 976-984   Apr 1997
This study tested the hypothesis that murine oocytes participate in the establishment of granulosa cell phenotypic heterogeneity in preovulatory follicles. In these follicles, mural granulosa cells express LH receptors (LHR) and LHR mRNA, but expr...
Yuji Hirao, Youki Tsuji, Takashi Miyano, Akira Okano, Masashi Miyake, Seishiro Kato, Robert M. Moor
Zygote   3 325-332   Jan 1995
The molecules involved in determining meiotic competence were determined in porcine oocytes isolated from preantral and antral follicles of different sizes. Oocytes isolated from preantral follicles had a mean diameter of 78 pm, contained diffuse ...
Takashi Miyano, Misa Ebihara, Yuri Goto, Yuji Hirao, Takashi Nagai, Seishiro Kato
Journal of Experimental Zoology   273 70-75   Jan 1995
The present study was conducted to investigate the direct action of hypoxanthine, which has been reported to be present in pig follicular fluid, on spontaneous meiotic resumption of pig denuded oocytes in vitro. No oocytes, which were surrounded b...
Y. Hirao, T. Nagai, M. Kubo, T. Miyano, M. Miyake, S. Kato
Journal of Reproduction and Fertility   100 333-339   Jan 1994
Preantral follicles containing oocytes of 70-89.5 μm in diameter were isolated from pig ovaries and cultured in collagen gel for up to 16 days, in the presence of serum, FSH and oestradiol. Formation of follicular antra occurred as the culture pro...
Yuji Hirao, Takashi Miyano, Seishiro Kato
Journal of Experimental Zoology   267 543-547   Jan 1993
The maturational competence of the in vitro grown mouse oocytes of various sizes was examined, and was compared to that of oocytes of equivalent chronological age which grew in vivo. Oocyte‐granulosa cell complexes isolated from 10‐day‐old female ...
Yuji Hirao, Junko Kimura, Takashi Miyan, Seishiro Kato
Journal of Reproduction and Development   39 13-17   Jan 1993
Ovaries from newborn female mice were cultured for 4 or 8 days in Eagle's minimum essential medium supplemented with pregnant mare's serum gonadotrophin (PMSG) at a concentration of 0, 2,10 or 50 lU/ml. At the beginning of culture, most oocytes in...
HIRAO Yuji, IKEDA Yoshi-hisa, MIYANO Takashi, KATO Seishiro
Journal of Mammalian Ova Research   10(2) 167-174   1993
Ovaries excised from 4-day-old mice were cultured for 20 days in the absence of gonadotrophins. In the ovaries, there were a great number of non-growing oocytes (10-19 μm in diameter) and a group of growing oocytes (20-39 μm). After organ culture,...
Y. Hirao, T. Miyano, S. Kato
Theriogenology   34 1071-1077   Jan 1990
Oocyte-granulosa cell complexes, isolated from 10-d-old mice, were cultured for 10 d in hypoxanthine-supplemented medium. The oocytes increased in size, but the resumption of meiosis was completely suppressed over the culture period. The oocytes w...
宮野隆, 平尾雄二, 加藤征史郎, 苅田淳
Anim Sci Technol   59(10) 848-853   Oct 1988
The early growth of mouse oocytes and the formation of zona pellucida in ovaries cultured in vitro were investigated histologically and compared to those in vivo. The ovaries excised from female mice at birth (Day 0) were cultured <i...
宮野隆, 平尾雄二, 加藤征史郎, 苅田淳
家畜繁殖学雑誌   34(1) 61-66   Mar 1988
The formation of the zona pellucida was studied histologically in relation to the oocyte growth in the mouse ovaries from at birth till 6 days after birth. No zona pellucida was found around the oocytes at birth. Patches of the zona pellucida were...

Conference Activities & Talks

有冨大輝, 尾畑やよい, 平尾雄二
日本卵子学会誌   1 Apr 2017   
的場理子, 平尾雄二, 武田久美子
食肉に関する助成研究調査成果報告書   Nov 2016   
諸白家奈子, 谷本連, 佐々木恵亮, 林克彦, 平尾雄二, 尾畑やよい
Journal of Reproduction and Development   Sep 2016   
谷本連, 諸白家奈子, 河野友宏, 平尾雄二, 尾畑やよい
Journal of Reproduction and Development   Sep 2016   
有冨大輝, 武田久美子, 尾畑やよい, 平尾雄二
Journal of Reproduction and Development   Sep 2016   
日下部央里絵, 浜崎伸彦, 永松剛, 尾畑やよい, 平尾雄二, 濱田律雄, 島本走, 今村拓也, 中島欽一, 斎藤通紀, 林克彦
Journal of Reproduction and Development   Sep 2016   
尾畑やよい, 平尾雄二
日本分子生物学会年会プログラム・要旨集(Web)   2016   
的場理子, 平尾雄二, 武田久美子
食肉に関する助成研究調査成果報告書   Nov 2015   
谷本連, 諸白家奈子, 河野友宏, 平尾雄二, 尾畑やよい
J Reprod Dev   5 Sep 2015   
尾畑やよい, 平尾雄二
J Reprod Dev   5 Sep 2015   
尾畑やよい, 平尾雄二
日本生殖医学会雑誌   1 Apr 2015   
農業・食品産業技術総合研究機構畜産草地研究所ニュース   Feb 2015   
諸白家奈子, 平尾雄二, 河野友宏, 尾畑やよい
J Reprod Dev   10 Aug 2014   
畜産技術   1 Jan 2014   
平尾雄二, CAO Feng, SOMFAI Tamas, 赤木悟史, 永井卓
J Mamm Ova Res   1 Apr 2013   
水町静華, 佐々木邦明, 松原和衛, 平尾雄二
J Reprod Dev   20 Aug 2012   
【目的】マウス卵母細胞の体外発育において2%ポリビニルピロリドン(PVP)の添加は透明帯の厚さを約30%減少させた (第104回大会)。本研究では,透明帯を挟んで連絡する卵母細胞と卵丘細胞の関係に着目し,それらの形態に及ぼすPVPの影響を検討した。【材料および方法】生後11~12日齢のマウス(ICR)の卵巣から前胞状卵胞を注射針で分離し,コラゲナーゼ処理した後,組織培養インサート上で培養した(37℃,5%CO2 in air,Day 0~10)。培養液には5%ウシ胎児...
HIRAO Yuji, NARUSE Kenji, KANEDA Masahiro, SOMFAI Tamas, IGA Kosuke, SHIMIZU Manabu, AKAGI Satoshi, NAGAI Takashi, TAKENOUCHI Naoki
Journal of mammalian ova research = 日本哺乳動物卵子学会誌   1 Apr 2012   
宮野隆, 平尾雄二
Horm Front Gynecol   1 Dec 2011   
【卵と卵胞の発育・成熟】 卵の発育と成熟 卵母細胞の発育制御機構
宮野 隆, 平尾 雄二
水町静華, 松原和衛, 平尾雄二
J Reprod Dev   20 Aug 2011   
平尾雄二, 成瀬健司, 伊賀浩輔, 竹之内直樹
J Mamm Ova Res   1 Apr 2011   
成瀬健司, 伊賀浩輔, 竹之内直樹, 志水学, 平尾雄二
J Reprod Dev   20 Aug 2010   
平尾雄二, 伊賀浩輔, 成瀬健司, 竹之内直樹
J Reprod Dev   20 Aug 2010   
竹鶴裕亮, 平尾雄二, 竹之内直樹, 伊賀浩輔, 宮野隆
日本畜産学会大会講演要旨   28 Mar 2010   
佐々木彩美, 松本浩樹, 川越雄太, 松原和衛, 平尾雄二
東北畜産学会報   5 Aug 2008   
Ex vivo growth and maturation of oocytes in farm animals
Miyano Takashi, Moniruzzaman Mohammad, Hirao Yuji, Manabe Noboru
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹
東北農業研究成果情報   Jun 2007   
J Mamm Ova Res   1 Apr 2007   
麻布大学雑誌   31 Mar 2007   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹
日本畜産学会大会講演要旨   27 Mar 2007   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹
畜産草地研究成果情報   25 Aug 2006   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹
東北農業研究成果情報   Aug 2006   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹
J Reprod Dev   25 Aug 2005   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹
J Mamm Ova Res   1 Apr 2005   
ブレインテクノニュース   30 Sep 2003   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹, 永井卓
東北農業研究成果情報   Sep 2003   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹, 永井卓
畜産草地研究成果情報   30 Aug 2003   
東北農業研究センターたより   Jul 2003   
平尾雄二, 伊藤丈洋, 志水学, 伊賀浩輔, 星宏良, 竹之内直樹
J Mamm Ova Res   1 Apr 2003   
平尾雄二, 志水学, 伊賀浩輔, 竹之内直樹
日本畜産学会大会講演要旨   20 Mar 2003   
平尾雄二, 相沢健一, 竹之内直樹, 永井卓
日本畜産学会大会講演要旨   20 Mar 2002   
Bing Y., 平尾 雄二, 竹之内 直樹, Che L., 永井 卓
日本胚移植学雑誌   Jan 2002   
永井卓, 平尾雄二, 竹之内直樹, 今井敬
東北農業研究成果情報   Aug 2001   
永井卓, 平尾雄二, 竹之内直樹, 今井敬
畜産研究成果情報   24 Mar 2001   
平尾雄二, 永井卓, 伊賀浩輔, 竹之内直樹
日本畜産学会大会講演要旨   20 Mar 2001   
西本凡子, 永井卓, 山内伸彦, 下司雅也, 竹之内直樹, 平尾雄二, 紅林賢臣, 桝田博司
Journal of Reproduction and Development   25 Dec 2000   
平尾雄二, 紅林賢臣, 竹之内直樹, 西本凡子, 永井卓
日本畜産学会大会講演要旨   20 Mar 2000   
平尾 雄二
日本畜産学会大会講演要旨集   Mar 1998   
原田真紀子, 平尾雄二, 宮野隆, 三宅正史, 加藤征史郎
家畜繁殖学会講演要旨   Sep 1994   
宮野隆, 平尾雄二, 加野浩一郎, 永井卓, 三宅正史, 加藤征史郎
産婦人科の世界   Mar 1994   
平尾雄二, 森竹貞宜, 辻雄貴, 宮野隆, 三宅正史, 加藤征史郎
日本畜産学会大会講演要旨   Mar 1994   
辻雄貴, 平尾雄二, 加野浩一郎, 宮野隆, 三宅正史, 加藤征史郎
日本畜産学会大会講演要旨   Mar 1994   
池田佳久, 平尾雄二, 宮野隆, 三宅正史, 加藤征史郎
日本畜産学会大会講演要旨   Mar 1994   
平尾雄二, 永井卓, 久保正法, 宮野隆, 三宅正史, 加藤征史郎
家畜繁殖学会講演要旨   Sep 1992   
平尾雄二, 宮野隆, 三宅正史, 加藤征史郎
日本畜産学会大会講演要旨   Mar 1992   
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