Yuji Hirao - My portal

Profile Information

Affiliation: Unit Leader, Embryo Production Research Unit, Division of Animal Breeding and Reproduction Research, National Agriculture and Food Research Organization

J-GLOBAL ID: 201601012869633650
researchmap Member ID: B000262803

External link: http://www.naro.affrc.go.jp/nilgs-neo/introduction/chart/0402/index.html

毎年、体外受精によって作られる受精卵からたくさんの牛が誕生していますが、卵巣内には受精卵になるための潜在的卵子がたくさん存在します。そのうち、短期間の培養で受精可能な卵子になるものはすでに利用されています。これまでに利用できた最も小さい卵子は、２週間の培養で受精能力を獲得させることに成功したものです。今後、さらに小さな卵子を利用するべく培養系の開発を進めています。

Research Interests

Research Areas

Life sciences / Animal production science / Reproductive biology

Research History

Apr, 2016 - Present

National Agriculture and Food Research Organization
Apr, 2012 - Mar, 2016

National Agriculture and Food Research Organization, National Institute of Livestock and Grassland Science
Apr, 2011 - Mar, 2012

National Agriculture and Food Research Organization, Tohoku Agricultural Research Center
Apr, 2006 - Mar, 2011

National Agriculture and Food Research Organization, Tohoku Agricultural Research Center
Apr, 2002 - Mar, 2006

主任研究官, 畜産草地部育種繁殖研究室, 独立行政法人農業技術研究機構東北農業研究センター
Apr, 2000 - Mar, 2001

Ministry of Agriculture, Forestry and Fisheries
Apr, 1999 - Mar, 2000

Ministry of Agriculture, Forestry and Fisheries
Oct, 1998 - Mar, 1999

Faculty of Agriculture, Kobe University
Jan, 1995 - Oct, 1998

ポスドク研究員, 米国ジャクソン研究所

Education

Apr, 1990 - Mar, 1993

Doctoral Course, The Graduate School of Science and Technology, Kobe University

Awards

May, 2017

Cozzarelli Prize, Complete in vitro generation of fertile oocytes from mouse primordial germ cells, The National Academy of Sciences of the United States of America

Kanako Morohaku, Ren Tanimoto, Keisuke Sasaki, Ryouka Kawahara-Miki, Tomohiro Kono, Katsuhiko Hayashi, Yuji Hirao, Yayoi Obata
Mar, 2015

Excellent Paper Award, Conditions affecting growth and developmental competence of mammalian oocytes in vitro, Animal Science Journal

Yuji Hirao
Sep, 2012

JRD Outstanding Paper Awards in 2012, Optimization of Oxygen Concentration for Growing Bovine Oocytes In Vitro: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes, The Journal of Reproduction and Development (JRD)

Yuj Hirao, Manabu Shimizu, Kosuke Iga, Naoki Takenouchi
Sep, 2011

The SRD Outstanding Research Award, Factors Affecting the Developmental Competence of Bovine and Porcine oocytes Grown In Vitro, Society for Reproduction and Development (SRD)

Yuji Hirao

Papers

Reconstitution of mouse oogenesis in a dish from pluripotent stem cells

Katsuhiko Hayashi, Orie Hikabe, Yayoi Obata, Yuji Hirao

NATURE PROTOCOLS, 12(9) 1733-1744, Sep, 2017 Peer-reviewed
Development of fertile mouse oocytes from mitotic germ cells in vitro

Kanako Morohaku, Yuji Hirao, Yayoi Obata

NATURE PROTOCOLS, 12(9) 1817-1829, Sep, 2017 Peer-reviewed
Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro

Kanako Morohaku, Yuji Hirao, Yayoi Obata

ANNALS OF BIOMEDICAL ENGINEERING, 45(7) 1608-1619, Jul, 2017 Peer-reviewed
Recent advances in understanding the regulation of oogenesis and its recapitulation in vitro: Mouse and bovine models

Yuji Hirao

Journal of Mammalian Ova Research, 34(1) 23-29, Apr 1, 2017
Reconstitution in vitro of the entire cycle of the mouse female germ line

Orie Hikabe, Nobuhiko Hamazaki, Go Nagamatsu, Yayoi Obata, Yuji Hirao, Norio Hamada, So Shimamoto, Takuya Imamura, Kinichi Nakashima, Mitinori Saitou …

NATURE, 539(7628) 299-+, Nov, 2016 Peer-reviewed
Complete in vitro generation of fertile oocytes from mouse primordial germ cells

Kanako Morohaku, Ren Tanimoto, Keisuke Sasaki, Ryouka Kawahara-Miki, Tomohiro Kono, Katsuhiko Hayashi, Yuji Hirao, Yayoi Obata

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(32) 9021-9026, Aug, 2016 Peer-reviewed
Developmental competence of oocytes grown in vitro: Has it peaked already?

Kanako Morohaku, Yuji Hirao, Yayoi Obata

JOURNAL OF REPRODUCTION AND DEVELOPMENT, 62(1) 1-5, Feb, 2016 Peer-reviewed
Synchronization of in vitro maturation in porcine oocytes

Tamás Somfai, Yuji Hirao

Methods in Molecular Biology, 1524 255-264, 2016 Peer-reviewed
大型動物における卵母細胞の体外発育

平尾雄二, 宮野隆

Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 31(3) 79-85, Oct, 2014
体外発育卵母細胞をレシピエントとしたクローン牛の誕生

平尾雄二

日本はい移植学雑誌, 36(2) 103-107, May, 2014
In vitro oocyte development in large animals

Yuji Hirao, Takashi Miyano

Journal of Mammalian Ova Research, 31(3) 79-85, 2014
In vitro growth and maturation of vitrified-warmed bovine oocytes collected from early antral follicles

Yuji Hirao, Tamás Somfai, Kenji Naruse

Journal of Reproduction and Development, 60(1) 68-72, 2014 Peer-reviewed
Production of Fertile Offspring from Oocytes Grown In Vitro by Nuclear Transfer in Cattle

Yuji Hirao, Kenji Naruse, Masahiro Kaneda, Tamas Somfai, Kosuke Iga, Manabu Shimizu, Satoshi Akagi, Feng Cao, Tomohiro Kono, Takashi Nagai …

BIOLOGY OF REPRODUCTION, 89(3) 57, Sep, 2013 Peer-reviewed
Effect of androstenedione on the growth and meiotic competence of bovine oocytes from early antral follicles

Hiroaki Taketsuru, Yuji Hirao, Naoki Takenouchi, Kosuke Iga, Takashi Miyano

ZYGOTE, 20(4) 407-415, Nov, 2012 Peer-reviewed
Milrinone Treatment of Bovine Oocytes During In Vitro Maturation Benefits Production of Nuclear Transfer Embryos by Improving Enucleation Rate and Developmental Competence

Kenji Naruse, Kosuke Iga, Manabu Shimizu, Naoki Takenouchi, Satoshi Akagi, Tamas Somfai, Yuji Hirao

JOURNAL OF REPRODUCTION AND DEVELOPMENT, 58(4) 476-483, Aug, 2012 Peer-reviewed
Optimization of Oxygen Concentration for Growing Bovine Oocytes In Vitro: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes

Yuji Hirao, Manabu Shimizu, Kosuke Iga, Naoki Takenouchi

JOURNAL OF REPRODUCTION AND DEVELOPMENT, 58(2) 204-211, Apr, 2012 Peer-reviewed
Isolation of Ovarian Components Essential for Growth and Development of Mammalian Oocytes In Vitro

Yuji Hirao

JOURNAL OF REPRODUCTION AND DEVELOPMENT, 58(2) 167-174, Apr, 2012 Peer-reviewed
Oocyte growth in vitro: Potential model for studies of oocyte-granulosa cell interactions

Yuji Hirao

Reproductive Medicine and Biology, 11(1) 1-9, 2012
Conditions affecting growth and developmental competence of mammalian oocytes in vitro

Yuji Hirao

ANIMAL SCIENCE JOURNAL, 82(2) 187-197, 2011 Peer-reviewed
Synchronization of in vitro maturation in porcine oocytes.

Somfai T, Hirao Y

Methods in molecular biology (Clifton, N.J.), 761 211-225, 2011 Peer-reviewed

Books and Other Publications

哺乳動物の発生工学

佐藤英明, 河野友宏, 内藤邦彦, 小倉淳郎, 尾畑やよい, 宮野隆, 平尾雄二, 種村健太郎, 柏崎直巳, 川原学, 濱野光市, 長嶋比呂志, 三谷匡, 徳永智之, 今井裕, 若山照彦, 高岸聖彦

朝倉書店, 2014 (ISBN: 9784254450293)

Presentations

マウス胎仔卵巣の器官培養におけるStem cell factorの添加が成熟卵子の体外作出に及ぼす影響

有冨大輝, 尾畑やよい, 平尾雄二

日本卵子学会誌, Apr 1, 2017
Studies of in vitro embryo production using growing oocytes collected from the ovaries removed from Japanese beef calves before fattening

的場理子, 平尾雄二, 武田久美子

食肉に関する助成研究調査成果報告書, Nov, 2016
マウス胎仔卵巣のガラス化保存と体外培養による始原生殖細胞の高度利用技術の開発

諸白家奈子, 谷本連, 佐々木恵亮, 林克彦, 平尾雄二, 尾畑やよい

Journal of Reproduction and Development, Sep, 2016
ステロイドホルモン受容体の制御がin vitroにおけるマウス卵胞形成に果たす役割

谷本連, 諸白家奈子, 河野友宏, 平尾雄二, 尾畑やよい

Journal of Reproduction and Development, Sep, 2016
マウス始原生殖細胞を成熟卵子へと誘導する新規培養系の開発

有冨大輝, 武田久美子, 尾畑やよい, 平尾雄二

Journal of Reproduction and Development, Sep, 2016
多能性幹細胞から卵母細胞を作出する体外培養技術の開発

日下部央里絵, 浜崎伸彦, 永松剛, 尾畑やよい, 平尾雄二, 濱田律雄, 島本走, 今村拓也, 中島欽一, 斎藤通紀, 林克彦

Journal of Reproduction and Development, Sep, 2016
マウス始原生殖細胞から卵子を産生する新規in vitro系の開発

尾畑やよい, 平尾雄二

日本分子生物学会年会プログラム・要旨集(Web), 2016
Production of embryos using growing oocytes derived from the ovaries removed bovine calves before fattening

的場理子, 平尾雄二, 武田久美子

食肉に関する助成研究調査成果報告書, Nov, 2015
in vitroで分化したマウス胎仔卵巣における網羅的遺伝子発現解析

谷本連, 諸白家奈子, 河野友宏, 平尾雄二, 尾畑やよい

J Reprod Dev, Sep 5, 2015
in vitroにおいて産生されたマウス卵の発生能

尾畑やよい, 平尾雄二

J Reprod Dev, Sep 5, 2015
in vitroにおける卵母細胞の成長・成熟

尾畑やよい, 平尾雄二

日本生殖医学会雑誌, Apr 1, 2015
体外でウシ卵子をつくる―卵母細胞の体外発育培養法の確立―

平尾雄二

農業・食品産業技術総合研究機構畜産草地研究所ニュース, Feb, 2015
新生仔マウス由来卵胞から体外培養で得られた卵の発生能解析

諸白家奈子, 平尾雄二, 河野友宏, 尾畑やよい

J Reprod Dev, Aug 10, 2014
ウシ卵母細胞の体外成熟改善技術

平尾雄二

畜産技術, Jan 1, 2014
ウシ体外発育・体外成熟卵母細胞の遺伝子発現プロファイル

平尾雄二, CAO Feng, SOMFAI Tamas, 赤木悟史, 永井卓

J Mamm Ova Res, Apr 1, 2013
体外発育培養液へのポリビニルピロリドン添加がマウス卵母細胞・透明帯・卵丘細胞の接点の微細形態に及ぼす影響

水町静華, 佐々木邦明, 松原和衛, 平尾雄二

J Reprod Dev, Aug 20, 2012
ウシ卵母細胞の体外での発育と成熟能力の獲得に及ぼすエストラジオール17βおよびアンドロステンジオンの影響

牧田美穂, 平尾雄二, 宮野隆

J Reprod Dev, Aug 20, 2012
Ability and characteristics of bovine nuclear transfer embryos reconstructed using cytoplasm derived from in vitro grown oocytes

HIRAO Yuji, NARUSE Kenji, KANEDA Masahiro, SOMFAI Tamas, IGA Kosuke, SHIMIZU Manabu, AKAGI Satoshi, NAGAI Takashi, TAKENOUCHI Naoki

Journal of mammalian ova research = 日本哺乳動物卵子学会誌, Apr 1, 2012
【卵と卵胞の発育・成熟】卵の発育と成熟卵母細胞の発育制御機構

宮野隆, 平尾雄二

HORMONE FRONTIER IN GYNECOLOGY, Dec, 2011
卵と卵胞の発育・成熟卵の発育と成熟(1)卵母細胞の発育制御機構

宮野隆, 平尾雄二

Horm Front Gynecol, Dec 1, 2011

Professional Memberships

Industrial Property Rights

特許第5435422号ウシの発情同期化法およびそのためのキット

竹之内直樹, 平尾雄二, 志水学, 伊賀浩輔
特開2012-210199 体外発育卵母細胞をレシピエント卵子とするクローン動物個体の作成法

平尾雄二, 伊賀浩輔, 成瀬健司, 竹之内直樹, 志水学
特許第4122425号卵母細胞の培養方法及び発育方法

平尾雄二