Sep, 2019
Acceleration of bone regeneration of horizontal bone defect in rats using collagen‐binding basic fibroblast growth factor combined with collagen scaffolds.
Journal of Periodontology
- Volume
- 90
- Number
- 9
- First page
- 1043
- Last page
- 1052
- Language
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1002/jper.18-0674
- Publisher
- Wiley
Background: Basic fibroblast growth factor (bFGF) has been applied for periodontal regeneration. However, the application depends on bone defect morphology because bFGF diffuses rapidly from defect sites. In a previous study, collagen-binding bFGF (CB-bFGF) has been shown to enhance bone formation by collagen-anchoring in the orthopedic field. The aim of this study is to demonstrate the efficacy of CB-bFGF with collagen scaffolds in bone regeneration of horizontal bone defect. Methods: Cell proliferation activity and collagen binding activity of CB-bFGF was confirmed by WST-8 assay and collagen binding assay, respectively. The retention of CB-bFGF in the collagen sheet (CS) was measured by fluorescence imaging. The rat horizontal alveolar bone defect model was employed to investigate the efficacy of CB-bFGF with collagen powder (CP). After 4 and 8 weeks, the regenerative efficacy was evaluated by microcomputed tomography, histological, and immunohistochemical analyses. Results: CB-bFGF had a comparable proliferation activity to bFGF and a collagen binding activity. CB-bFGF was retained in CS longer than bFGF. At 8 weeks postoperation, bone volume, bone mineral content, and new bone area in CB-bFGF/CP group were significantly increased compared with those in other groups. Furthermore, epithelial downgrowth was significantly suppressed in CB-bFGF/CP group. At 4 weeks, the numbers of osteocalcin, proliferating cell nuclear antigen, and osteopontin-positive cells at the regeneration site in CB-bFGF/CP group were greater than those in other groups. Conclusions: CB-bFGF/CP effectively promoted bone regeneration of horizontal bone defect possibly by sustained release of bFGF. The potential of CB-bFGF composite material for improved periodontal regeneration in vertical axis was shown.
- Link information
-
- DOI
- https://doi.org/10.1002/jper.18-0674
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30889294
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850180
- URL
- https://onlinelibrary.wiley.com/doi/pdf/10.1002/JPER.18-0674
- URL
- https://onlinelibrary.wiley.com/doi/full-xml/10.1002/JPER.18-0674
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85068083606&origin=inward Open access
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85068083606&origin=inward
- ID information
-
- DOI : 10.1002/jper.18-0674
- ISSN : 0022-3492
- eISSN : 1943-3670
- Pubmed ID : 30889294
- Pubmed Central ID : PMC6850180
- SCOPUS ID : 85068083606