In this study, we focused on the development of a novel biomimetic vascular graft purposing application for congenital heart disease. For this research period, we had made the reasonable vascular scaffold, followed by decellularization of rat-derived intestinal tissue. However, although most critical issue in this project was to achieve the improved recellularization, we could not have a successful cell attachment to the scaffold. In addition, we tried to increasing cell number in the scaffold by use of collagen-anchoring original technology, unfortunately, resulted in unchanged cell engraftment. In conclusion, we could not reach an initial goal, however, some technical issues regarding improved cell engraftment such as adopting other growth factor were clarified. Therefore, we might continue the investigation for creating a new device, to improve the prognosis of the patients with congenital heat disease.