2012年5月
West Nile virus noncoding subgenomic RNA contributes to viral evasion of the type I interferon-mediated antiviral response
Journal of Virology
- 巻
- 86
- 号
- 10
- 開始ページ
- 5708
- 終了ページ
- 5718
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1128/JVI.00207-12
- 出版者・発行元
- AMER SOC MICROBIOLOGY
We previously showed that a noncoding subgenomic flavivirus RNA (sfRNA) is required for viral pathogenicity, as a mutant West Nile virus (WNV) deficient in sfRNA production replicated poorly in wild-type mice. To investigate the possible immunomodulatory or immune evasive functions of sfRNA, we utilized mice and cells deficient in elements of the type I interferon (IFN) response. Replication of the sfRNA mutant WNV was rescued in mice and cells lacking interferon regulatory factor 3 (IRF-3) and IRF-7 and in mice lacking the type I alpha/beta interferon receptor (IFNAR), suggesting a contribution for sfRNA in overcoming the antiviral response mediated by type I IFN. This was confirmed by demonstrating rescue of mutant virus replication in the presence of IFNAR neutralizing antibodies, greater sensitivity of mutant virus replication to IFN-α pretreatment, partial rescue of its infectivity in cells deficient in RNase L, and direct effects of transfected sfRNA on rescuing replication of unrelated Semliki Forest virus in cells pretreated with IFN-α. The results define a novel function of sfRNA in flavivirus pathogenesis via its contribution to viral evasion of the type I interferon response. © 2012, American Society for Microbiology.
- リンク情報
-
- DOI
- https://doi.org/10.1128/JVI.00207-12
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22379089
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000303787100027&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84861312740&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84861312740&origin=inward
- ID情報
-
- DOI : 10.1128/JVI.00207-12
- ISSN : 0022-538X
- eISSN : 1098-5514
- PubMed ID : 22379089
- SCOPUS ID : 84861312740
- Web of Science ID : WOS:000303787100027