論文

査読有り
2015年9月

Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism

MICROBIAL BIOTECHNOLOGY
  • Kyohei Baito
  • ,
  • Satomi Imai
  • ,
  • Makoto Matsushita
  • ,
  • Miku Otani
  • ,
  • Yu Sato
  • ,
  • Hiroyuki Kimura

8
5
開始ページ
837
終了ページ
845
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/1751-7915.12179
出版者・発行元
WILEY-BLACKWELL

In a deep aquifer associated with an accretionary prism, significant methane (CH4) is produced by a subterranean microbial community. Here, we developed bioreactors for producing CH4 and hydrogen (H-2) using anaerobic groundwater collected from the deep aquifer. To generate CH4, the anaerobic groundwater amended with organic substrates was incubated in the bioreactor. At first, H-2 was detected and accumulated in the gas phase of the bioreactor. After the H-2 decreased, rapid CH4 production was observed. Phylogenetic analysis targeting 16S rRNA genes revealed that the H-2-producing fermentative bacterium and hydrogenotrophic methanogen were predominant in the reactor. The results suggested that syntrophic biodegradation of organic substrates by the H-2-producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH4 production. For H-2 production, the anaerobic groundwater, amended with organic substrates and an inhibitor of methanogens (2-bromoethanesulfonate), was incubated in a bioreactor. After incubation for 24h, H-2 was detected from the gas phase of the bioreactor and accumulated. Bacterial 16S rRNA gene analysis suggested the dominance of the H-2-producing fermentative bacterium in the reactor. Our study demonstrated a simple and rapid CH4 and H-2 production utilizing anaerobic groundwater containing an active subterranean microbial community.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1111/1751-7915.12179
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25267392
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000359607200008&DestApp=WOS_CPL