2017年4月7日
Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices.
Scientific reports
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- 巻
- 7
- 号
- 開始ページ
- 46213
- 終了ページ
- 46213
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep46213
- 出版者・発行元
- NATURE PUBLISHING GROUP
Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial activity and glycolysis, we simultaneously recorded sperm motility and enzymatic reactivity using a portable motility analysis system (iSperm) and a paper-based MTT assay, respectively. When using our paper-based MTT-assay, we calculated the area mean value signal intensity (AMV) to evaluate enzymatic reactivity. Both sperm motility and AMV decreased following treatment with iodoacetamide (IODO) and 3-bromopyruvic acid (3BP), both of which are inhibitors of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found a correlation between recorded motility using iSperm and AMV from our paper-based assay (P < 0.05), suggesting that a sperm-related enzymatic reaction is involved in sperm motility. Under this protocol, MTT reduction was coupled with catalysis of GAPDH and was promoted by electron transfer from NADH. Based on this inhibitor study, sperm motility can be estimated using our paper-based MTT-assay.
- リンク情報
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- DOI
- https://doi.org/10.1038/srep46213
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28387379
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384208
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000398638400001&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1038/srep46213
- ISSN : 2045-2322
- PubMed ID : 28387379
- PubMed Central 記事ID : PMC5384208
- Web of Science ID : WOS:000398638400001