2015
Modification of the cell adhesion and hydrophilic characteristics of poly(ether-ether-ketone) by 172-nm Xenon excimer radiation
BIO-MEDICAL MATERIALS AND ENGINEERING
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- Volume
- 25
- Number
- 2
- First page
- 169
- Last page
- 175
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.3233/BME-151267
- Publisher
- IOS PRESS
BACKGROUND: Poly-ether-ether-ketone (PEEK) has biomechanical and chemical properties that are excellent for biomedical applications; however, PEEK adhesion to bone or chondral tissue proceeds slowly due to poor hydrophilicity and other surface characteristics.
OBJECTIVE: We investigated the structural change, hydrophilicity, and cytocompatibility of a PEEK surface after 172-nm xenon excimer UV-irradiation.
METHODS: The surface characteristics before and after irradiation were evaluated by contact angle and ATR-FTIR measurements. Mouse osteoblast-like cells (MC3T3-E1) were cultured on PEEK plates and collected after 6, 12 and 24 h for cell adhesion analysis by crystal violet staining (CVS) and scanning electron microscopy (SEM).
RESULTS: UV-irradiation improved PEEK surface hydrophilicity, as indicated by a significant drop in water contact angle (p < 0.05). Irradiated PEEK showed additional peaks around 3370 cm(-1) and 1720 cm(-1), highlighting the generation of hydroxyl and carbonyl groups. CVS and SEM revealed improved adhesion to the PEEK surface after UV-irradiation.
CONCLUSION: Our results suggest that 172-nm UV-irradiated PEEK may be used in biomedical applications that require good cell adhesion.
OBJECTIVE: We investigated the structural change, hydrophilicity, and cytocompatibility of a PEEK surface after 172-nm xenon excimer UV-irradiation.
METHODS: The surface characteristics before and after irradiation were evaluated by contact angle and ATR-FTIR measurements. Mouse osteoblast-like cells (MC3T3-E1) were cultured on PEEK plates and collected after 6, 12 and 24 h for cell adhesion analysis by crystal violet staining (CVS) and scanning electron microscopy (SEM).
RESULTS: UV-irradiation improved PEEK surface hydrophilicity, as indicated by a significant drop in water contact angle (p < 0.05). Irradiated PEEK showed additional peaks around 3370 cm(-1) and 1720 cm(-1), highlighting the generation of hydroxyl and carbonyl groups. CVS and SEM revealed improved adhesion to the PEEK surface after UV-irradiation.
CONCLUSION: Our results suggest that 172-nm UV-irradiated PEEK may be used in biomedical applications that require good cell adhesion.
- Link information
- ID information
-
- DOI : 10.3233/BME-151267
- ISSN : 0959-2989
- eISSN : 1878-3619
- Web of Science ID : WOS:000351738200006