1995年4月
A GENE ENCODING A PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C IS INDUCED BY DEHYDRATION AND SALT STRESS IN ARABIDOPSIS-THALIANA
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- ,
- ,
- ,
- 巻
- 92
- 号
- 9
- 開始ページ
- 3903
- 終了ページ
- 3907
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.92.9.3903
- 出版者・発行元
- NATL ACAD SCIENCES
A cDNA corresponding to a putative phosphatidylinositol-specific phospholipase C (PI-PLC) in the higher plant Arabidopsis thaliana was cloned by use of the polymerase chain reaction, The cDNA, designated cAtPLC1, encodes a putative polypeptide of 561 aa with a calculated molecular mass of 64 kDa. The putative product includes so-called X and Y domains found in all PI-PLCs identified to date. In mammalian cells, there are three types of PI-PLC, PLC-beta, -gamma, and -delta. The overall structure of the putative AtPLC1 protein is most similar to that of PLC-delta, although the AtPLC1 protein is much smaller than PLCs from other organisms. The recombinant AtPLC1 protein synthesized in Escherichia coli was able to hydrolyze phosphatidylinositol 4,5-bisphosphate and this activity was completely dependent on Ca2+, as observed also for mammalian PI-PLCs. These results suggest that the AtPLC1 gene encodes a genuine PI-PLC of a higher plant. Northern blot analysis showed that the AtPLC1 gene is expressed at very low levels in the plant under normal conditions but is induced to a significant extent under various environmental stresses, such as dehydration, salinity, and low temperature, These observations suggest that AtPLC1 might be involved in the signal-transduction pathways of environmental stresses and that an increase in the level of AtPLC1 might amplify the signal, in a manner that contributes to the adaptation of the plant to these stresses.
- リンク情報
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- DOI
- https://doi.org/10.1073/pnas.92.9.3903
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1995QV93000055&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-0029061510&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0002-3868-2380
- ID情報
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- DOI : 10.1073/pnas.92.9.3903
- ISSN : 0027-8424
- ORCIDのPut Code : 17311997
- SCOPUS ID : 0029061510
- Web of Science ID : WOS:A1995QV93000055