2020年12月
The barley pan-genome reveals the hidden legacy of mutation breeding
Nature
- 巻
- 588
- 号
- 7837
- 開始ページ
- 284
- 終了ページ
- 289
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41586-020-2947-8
- 出版者・発行元
- Springer Science and Business Media LLC
<title>Abstract</title>Genetic diversity is key to crop improvement. Owing to pervasive genomic structural variation, a single reference genome assembly cannot capture the full complement of sequence diversity of a crop species (known as the ‘pan-genome’1). Multiple high-quality sequence assemblies are an indispensable component of a pan-genome infrastructure. Barley (<italic>Hordeum vulgare</italic> L.) is an important cereal crop with a long history of cultivation that is adapted to a wide range of agro-climatic conditions2. Here we report the construction of chromosome-scale sequence assemblies for the genotypes of 20 varieties of barley—comprising landraces, cultivars and a wild barley—that were selected as representatives of global barley diversity. We catalogued genomic presence/absence variants and explored the use of structural variants for quantitative genetic analysis through whole-genome shotgun sequencing of 300 gene bank accessions. We discovered abundant large inversion polymorphisms and analysed in detail two inversions that are frequently found in current elite barley germplasm; one is probably the product of mutation breeding and the other is tightly linked to a locus that is involved in the expansion of geographical range. This first-generation barley pan-genome makes previously hidden genetic variation accessible to genetic studies and breeding.
- リンク情報
- ID情報
-
- DOI : 10.1038/s41586-020-2947-8
- ISSN : 0028-0836
- eISSN : 1476-4687
- ORCIDのPut Code : 85430757
- SCOPUS ID : 85096565099