論文

査読有り 国際誌
2020年3月30日

Elevated levels of proinflammatory volatile metabolites in feces of high fat diet fed KK-Ay mice.

Scientific reports
  • Misaki Uchikawa
  • ,
  • Mai Kato
  • ,
  • Akika Nagata
  • ,
  • Shunsuke Sanada
  • ,
  • Yuto Yoshikawa
  • ,
  • Yuta Tsunematsu
  • ,
  • Michio Sato
  • ,
  • Takuji Suzuki
  • ,
  • Tsutomu Hashidume
  • ,
  • Kenji Watanabe
  • ,
  • Yuko Yoshikawa
  • ,
  • Noriyuki Miyoshi

10
1
開始ページ
5681
終了ページ
5681
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-62541-7

When the microfloral composition deteriorates, it triggers low-level chronic inflammation associated with several lifestyle-related diseases including obesity and diabetic mellitus. Fecal volatile organic compounds (VOCs) have been found to differ in gastrointestinal diseases as well as intestinal infection. In this study, to evaluate a potential association between the pathogenesis of lifestyle-related diseases and VOCs in the intestinal tract, fecal VOCs from obese/diabetic KK-Ay mice (KK) or controls (C57BL/6J mice; BL) fed a normal or high fat diet (NFD or HFD) were investigated using headspace sampler-GC-EI-MS. Principal component analysis (PCA) of fecal VOC profiles clearly separated the experimental groups depending on the mouse lineage (KK vs BL) and the diet type (NFD vs HFD). 16 s rRNA sequencing revealed that the PCA distribution of VOCs was in parallel with the microfloral composition. We identified that some volatile metabolites including n-alkanals (nonanal and octanal), acetone and phenol were significantly increased in the HFD and/or KK groups. Additionally, these volatile metabolites induced proinflammatory activity in the RAW264 murine macrophage cell line indicating these bioactive metabolites might trigger low-level chronic inflammation. These results suggest that proinflammatory VOCs detected in HFD-fed and/or diabetic model mice might be novel noninvasive diagnosis biomarkers for diabetes.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-62541-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32231228
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105489
ID情報
  • DOI : 10.1038/s41598-020-62541-7
  • PubMed ID : 32231228
  • PubMed Central 記事ID : PMC7105489

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