2020年7月1日
An immuno-dot blot assay for screening histidine kinase inhibitors
ANALYTICAL BIOCHEMISTRY
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- 巻
- 600
- 号
- 開始ページ
- 113765
- 終了ページ
- 113765
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ab.2020.113765
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Two-component signal transduction systems (TCSs), consisting of a histidine kinase (HK) and its cognate response regulator, are ubiquitous among bacteria and are associated with the virulence of pathogens. TCSs are potential targets for alternative antibiotics and antivirulence agents. It is, thus, very important to determine HK activity in bacterial TCSs. Here, we describe an immuno-dot blot assay for the inhibition profiling of HKs using the anti-N3-phosphohistidine antibody. This simple method promises reliable detection of HK activity, and it is likely applicable in high-throughput screening of HK inhibitors.
- ID情報
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- DOI : 10.1016/j.ab.2020.113765
- ISSN : 0003-2697