論文

2015年11月

Programmed Cell Death Progresses Differentially in Epidermal and Mesophyll Cells of Lily Petals

PLOS ONE
  • Hiroko Mochizuki-Kawai
  • ,
  • Tomoko Niki
  • ,
  • Kenichi Shibuya
  • ,
  • Kazuo Ichimura

10
11
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0143502
出版者・発行元
PUBLIC LIBRARY SCIENCE

In the petals of some species of flowers, programmed cell death (PCD) begins earlier in mesophyll cells than in epidermal cells. However, PCD progression in each cell type has not been characterized in detail. We separately constructed a time course of biochemical signs and expression patterns of PCD-associated genes in epidermal and mesophyll cells in Lilium cv. Yelloween petals. Before visible signs of senescence could be observed, we found signs of PCD, including DNA degradation and decreased protein content in mesophyll cells only. In these cells, the total proteinase activity increased on the day after anthesis. Within 3 days after anthesis, the protein content decreased by 61.8%, and 22.8% of mesophyll cells was lost. A second peak of proteinase activity was observed on day 6, and the number of mesophyll cells decreased again from days 4 to 7. These biochemical and morphological results suggest that PCD progressed in steps during flower life in the mesophyll cells. PCD began in epidermal cells on day 5, in temporal synchrony with the time course of visible senescence. In the mesophyll cells, the KDEL-tailed cysteine proteinase (LoCYP) and S1/P1 nuclease (LoNUC) genes were upregulated before petal wilting, earlier than in epidermal cells. In contrast, relative to that in the mesophyll cells, the expression of the SAG12 cysteine proteinase homolog (LoSAG12) drastically increased in epidermal cells in the final stage of senescence. These results suggest that multiple PCD-associated genes differentially contribute to the time lag of PCD progression between epidermal and mesophyll cells of lily petals.


リンク情報
DOI
https://doi.org/10.1371/journal.pone.0143502
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702221119418233
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365865300072&DestApp=WOS_CPL
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84960156315&origin=inward
ID情報
  • DOI : 10.1371/journal.pone.0143502
  • ISSN : 1932-6203
  • J-Global ID : 201702221119418233
  • SCOPUS ID : 84960156315
  • Web of Science ID : WOS:000365865300072

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