2015年3月
Polycistronic expression of RNA silencing suppressor protects its own mRNA from RNA silencing
PLANT BIOTECHNOLOGY
- ,
- ,
- 巻
- 32
- 号
- 1
- 開始ページ
- 89
- 終了ページ
- 95
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.5511/plantbiotechnology.15.0120b
- 出版者・発行元
- JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
The internal ribosome entry site (IRES) enables polycistronic expression of multiple genes from a single mRNA controlled by one promoter. In general, only the most 5' coding sequence is abundantly translated from polycistronic mRNAs in eukaryotic cells; IRES-mediated translation allows additional coding sequences at other positions to be translated at detectable levels. However, IRES-mediated translation often results in much lower protein expression than translation of single-gene mRNAs. We first aimed to improve IRES-mediated gene expression with a transient expression system based on Nicotiana benthamiana. We demonstrated that the presence of two cassettes comprised of an IRES-mediated Venus coding sequence results in higher Venus expression than one cassette. The double IRES cassette system is expected to be useful for expressing a gene of interest polycistronically in plants. Using the double IRES cassette system, we found that polycistronic expression of a reporter gene and two copies of an IRES-mediated RNA silencing suppressor gene protected the transcribed mRNA from RNA silencing-mediated degradation. This is the first report of a mRNA self-protection system from RNA silencing by IRES-mediated expression of a viral suppressor in plants.
- リンク情報
- ID情報
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- DOI : 10.5511/plantbiotechnology.15.0120b
- ISSN : 1342-4580
- eISSN : 1347-6114
- Web of Science ID : WOS:000353555700010